Drosophila melanogaster
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA118861 | ENA |
REPOSITORIES: ENA
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Project description:The systemic response to injury in Drosophila melanogaster is characterized by the activation of specific signaling pathways that facilitate the regeneration of wounded tissue and help coordinate wound healing with organism growth. The mechanisms by which damaged tissues influence the development and function of peripheral non-injured tissues is not fully understood. Injury was induced in early third instar larvae via temperature-dependent cell death in wing imaginal discs. Microarray analysis using RNA isolated from injured and control was used to identify genes underlying the systemic injury response. We identified 150 genes which were differentially expressed in response to localized cell death in wing imaginal discs. Upregulated genes were associated biological processes including carnitine biosynthesis, signal transduction and regulation of oxidoreductase activity while terms associated with downregulated genes included wound healing, imaginal disc-derived wing hair outgrowth, and regulation of glutamatergic synaptic transmission. Pathway analysis revealed that wing disc damage led to changes in fatty acid, cysteine, and carnitine metabolism. One gene, 14-3-3ζ, which encodes a known regulator of Ras/MAPK signaling was identified as a potential regulator of transdetermination during tissue regeneration. Our results raise the possibility that immune function and cell proliferation during wing disc repair and regeneration in Drosophila may require the sulfur amino acid cysteine and its’ metabolites, taurine and glutathione, similar to what has been reported during tissue repair in mammals. Further, it seems likely that imaginal disc damage stimulates the mobilization of fatty acids to support the energetically demanding process of tissue regeneration. The roles of additional genes that are differentially regulated following imaginal disc injury remain to be elucidated.
2017-09-30 | GSE94938 | GEO
Project description:We have carried out eukaryotic whole-genome Illumina RNA-seq of regenerating blastema cells and control undamaged wing imaginal disc cells to identify the differentially expressed genes during regeneration.
2017-07-25 | GSE101797 | GEO
Project description:Microarray comparison of anterior and posterior Drosophila wing imaginal disc cells
| PRJNA201243 | ENA
Project description:Mechanisms of tumor-cells-invasion in Drosophila wing imaginal disc epithelia
| PRJDB12759 | ENA
Project description:Sorted wing disc proneural cluster cells
| PRJNA91205 | ENA
Project description:The discovery of functional long non-coding RNAs (lncRNAs) changed the initial concept of lncRNAs as transcriptional noise. Since then, a wide array of functions have been associated with lncRNAs, including changes in the chromatin state and gene expression, modulation of splicing, translation or degradation of mRNAs. Also, many lncRNAs are known to be deregulated in multiple diseases, including cancer and neurological disorders. However, functional studies of lncRNAs are hindered by the usual lack of phenotypes upon deletion or inhibition. Here, we used Drosophila imaginal discs as a model system to identify putatively functional lncRNAs involved in development and regeneration. We described the subset of lncRNAs expressed in the wing, eye and leg disc development. Also, we analyzed transcriptomic data from regenerating wing discs to profile the expression pattern of lncRNAs upon damage. Particularly, we focused on the intergenic lncRNA CR40469 whose expression is upregulated in regeneration. We generated fully viable CR40469 mutants that showed no visible developmental defects. However, these mutants experience a remarkable loss of their wing regeneration capacity upon the induction of cell death. Additionally, we describe a duplication of the CR40469 genomic sequence in the Drosophila genome, but it does not seem to be necessary for development or wing regeneration.
2024-08-21 | GSE223411 | GEO
Project description:Drosophila melanogaster Micro-C maps on wing imaginal disc, larval brain and adult brain
| PRJNA885423 | ENA
Project description:Transcriptional profiling of Drosophila wing pouch following CR32027 RNAi
| PRJNA238066 | ENA
Project description:Gene expression changes in Nicd induced wing disc hyperplasia
| PRJNA176934 | ENA