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LIS1 promotes perineural invasion by upregulating the serine metabolism in head and neck squamous carcinoma

ABSTRACT: LIS1 promotes perineural invasion by upregulating the serine metabolism in head and neck squamous carcinoma

PROVIDER: PRJNA1190471 | ENA |

REPOSITORIES: ENA

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LIS1 promotes perineural invasion by upregulating the serine metabolism in head and neck squamous carcinoma

Project description:LIS1 is a critical mediator of neuronal migration during brain development, and is strongly associated with malignant tumors. We found that LIS1 is highly expressed in HNSCC,which is releated to perineural infiltration(PNI) in HNSCC.To explore the mechanism of PNI, we silenced the LIS1 expression in HNSCC cells and dected the function of PNI.In a word,We sought to validate LIS1 as a key signaling molecule for PNI in HNSCC.

2025-01-01 | GSE282822 | GEO

Project description:LIS1 promotes perineural invasion by upregulating the serine metabolism in head and neck squamous carcinoma

| PRJNA1189856 | ENA

Hepatic loss of Lis1 leads to fatty liver and predisposes to tumorigenesis in mice

Project description:We found that genetic deletion of Lis1 (also known as Pafah1b1) in mouse liver led to increased lipid accumulation and inflammation in the liver. Further analysis revealed that loss of Lis1 led to endoplasmic reticulum (ER) stress and reduced triglyceride secretion. Attenuation of ER stress by tauroursodeoxycholic acid (TUDCA) diminished lipid accumulation in the Lis1 defecient hepatocytes. In addition, Golgi was disorganized in Lis1 deficient livers. These mutants exhibited increased hepatocyte ploidy and accelerated development of liver cancer when treated with DEN. Taken together, these findings suggest that Lis1 is involved in the development and progression of liver diseases from steatosis to liver cancer and provide a useful model for delineating the molecular pathways that lead to these diseases.

2018-02-15 | GSE108096 | GEO

LIS1 RNA-binding orchestrates the mechanosensitive properties of embryonic stem cells in AGO2-dependent and independent ways

Project description:Lissencephaly-1 (LIS1) is associated with neurodevelopmental diseases and is known to regulate the molecular motor cytoplasmic dynein activity. Here we show that LIS1 is essential for the viability of mouse embryonic stem cells (mESCs), and it governs the physical properties of these cells. LIS1 dosage substantially affects gene expression, and we uncovered an unexpected interaction of LIS1 with RNA and RNA-binding proteins, most prominently the Argonaute complex. We demonstrate that LIS1 overexpression partially rescued the extracellular matrix (ECM) expression and mechanosensitive genes conferring stiffness to Argonaute null mESCs. Collectively, our data transforms the current perspective on the roles of LIS1 in post-transcriptional regulation underlying development and mechanosensitive processes.

2023-05-04 | GSE198390 | GEO

Transient knockdown of LIS1 results in seizures, differential gene expression and a neuronal migration disorder in developing zebrafish

Project description:Mutation or deletion of LIS1 (Lissencephaly-1) underlies classical lissencephaly, a migration disorder resulting in brain malformation, epilepsy and mental retardation. Orthologues for LIS1 genes (lis1a and lis1b) are ubiquitously expressed in developing zebrafish larvae, but the functional consequences of Lis1 knockdown are unknown. Here we used lis1-specific morpholino oligonucleotides (MOs, targeting protein translation or mRNA splicing, respectively) to transiently knockdown Lis1 expression in zebrafish. Injection of lis1 MOs resulted in morphological changes including microcephaly. At four days post-fertilization, lis1 morphants exhibited spontaneous convulsive behavior and abnormal large-amplitude electrographic discharge resembling that seen in acute and genetic zebrafish models of epilepsy. Abnormal brain development and neuronal migration defects were observed when lis1 MO injections were made into fluorescent reporter lines demarcating interneuron distribution (Dlx5a/6a:GFP) and brain structure (GBT0133:mRFP). Microarray analysis for ~44,000 Danio rerio transcripts identified 215 up-regulated and 160 down-regulated genes. Quantitative PCR and whole-mount in situ hybridization were used to validate these results for twenty and seven genes, respectively. These findings in a simple vertebrate model reproducing neuroanatomical and epileptic hallmarks of the human condition represent a novel approach to the study of childhood seizure disorders associated with a single gene mutation 4 WT samples (sample= 10 pooled larvae) and 4 Lis1Morphants slight phenotype (sample= 10 pooled larvae) were collected at 4dpf (days post fertilization). The morphants were selected based on phenotype and seizure behavior. Both groups of fish derived from same pools of eggs

2013-06-15 | E-GEOD-47952 | biostudies-arrayexpress

Transient knockdown of LIS1 results in seizures, differential gene expression and a neuronal migration disorder in developing zebrafish

2013-06-15 | GSE47952 | GEO

LIS1 RNA binding orchestartes the mechanosensitive properties of embryonic stem cells in AGO2 dependent and independent ways.

Project description:LIS1 immunoprecipitation in nuclear and cytoplasmic fractions of LIS1 Floxed/-, OE and WT embryonic stem cells. To identify AGO2 dependent and independent interactions LIS1 was immunoprecipitated in AGO1-4 knockout and doxycycline induced AGO2 mouse embryonic stem cells.

2023-07-20 | PXD033150 | Pride

Requirement for Lis1 in normal and malignant hematopoietic stem cell renewal to investigate requirement for Lis1 in normal and malignant hematopoietic stem cell renewal

Project description:Adult Lis1+/+creER or Lis1f/fcreER mice were treated with tamoxifen for five consecutive days. Three days after the final tamoxifen administration, KLS (ckit+ Lin- Sca1+) bone marrow cells were FACS-sorted and total cellular RNAs were purified. RNAs were amplified, labeled, hybridized onto Affymetrix GeneChip Mouse Genome 430 2.0 Arrays and raw hybridization data were collected.

2014-02-02 | E-MEXP-3855 | biostudies-arrayexpress

LIS1 RNA-binding orchestrates the mechanosensitive properties of embryonic stem cells in AGO2-dependent and independent ways [2_MARS_seq_B]

Project description:We analyzed LIS1 dosage dependent gene expression changes in human embryonic stem cells.

2023-05-04 | GSE198343 | GEO

LIS1 RNA-binding orchestrates the mechanosensitive properties of embryonic stem cells in AGO2-dependent and independent ways [1_MARS_seq_A]

Project description:We analyzed LIS1 dosage dependent gene expression changes in mouse and human embryonic stem cells.

2023-05-04 | GSE198342 | GEO