Cryphia algae
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1191608 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| ERR13946465.fastq.gz | Fastqsanger.gz | |||
| ERR13947485_1.fastq.gz | Fastqsanger.gz | |||
| ERR13947485_2.fastq.gz | Fastqsanger.gz |
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Project description:Shewanella algae (S. algae), an emerging opportunistic pathogen, has been increasingly associated with severe gastrointestinal infections, particularly among immunocompromised individuals, including those with colorectal adenomas. To evaluate its potential role in the progression of colorectal adenomas, we conducted both in vivo and in vitro studies. In the in vivo model, mice bearing adenomas were orally administered S. algae to simulate natural ingestion. Concurrently, macrophages were exposed to S. algae in vitro to assess cellular responses. Oral administration of S. algae was found to exacerbate intestinal inflammation and increase colorectal adenoma burden. Transcriptomic analyses revealed elevated levels of reactive oxygen species (ROS) and upregulated expression of acyl-CoA synthetase long-chain family member 4 (Acsl4), indicative of enhanced iron metabolism and lipid peroxidation, key features of ferroptosis. Furthermore, the expression of key ferroptosis suppressors, including glutathione peroxidase 4 (Gpx4) and solute carrier family 7 member 11 (Slc7a11), was significantly downregulated. These pathological changes were effectively reversed upon treatment with ferrostatin-1 (Fer-1), a specific inhibitor of ferroptosis. In vitro experiments corroborated the in vivo findings, demonstrating consistent activation of ferroptotic pathways in macrophages. Collectively, these results suggest that S. algae may promote the progression of colorectal adenomas by inducting ferroptosis in both colonic tissues and macrophages. Therefore, therapeutic strategies targeting ferroptosis may offer a promising approach for managing high-risk individuals infected with S. algae, potentially improving clinical outcomes and quality of life.
2026-08-30 | GSE345022 | GEO
Project description:Cypermethrin (CYP) is one of the most widely used pesticides in large scale for agricultural and domestic purpose and the residue often seriously affects aquatic system. Environmental pollutants induced protein changes in organisms could be detected by proteomics, leading to discovery of potential biomarkers and understanding of mode of action. While proteomics investigations of CYP stress in some animal models have been well studied, few reports about the effects of exposure to CYP on algae proteome were published. To determine CYP effect in algae, the impact of various dosages (0.001 µg/L, 0.01 µg/L and 1 µg/L) of CYP on green algae Chlorella Vulgaris for 24h and 96h were investigated by using iTRAQ quantitative proteomics technique. A total of 162 and 198 proteins were significantly altered after CYP exposure for 24h and 96h, respectively. Overview of iTRAQ results indicated that the influence of CYP on algae protein might be dosage-dependent. Functional analysis of differentially expressed proteins showed that CYP could induce protein alterations related to photosynthesis, stress responses and carbohydrates metabolism. This study provides a comprehensive view of complex mode of action of algae under CYP stress and highlights several potential biomarkers for further investigation of pesticides exposed plant and algae.
2016-03-14 | PXD003521 | Pride
Project description:Shewanella spp. possess a broad respiratory versatility, which contributes to the occupation of hypoxic/anoxic environmental or host-associated niches. Here we observed a strain-specific induction of biofilm formation in response to supplementation with the anaerobic electron acceptors dimethyl sulfoxide (DMSO) and nitrate in a panel of Shewanella algae isolates. The respiration-driven biofilm response is not observed in DMSO and nitrate reductase deletion mutants of the type strain S. algae CECT 5071, and can be restored upon complementation with the corresponding reductase operon(s) but not by an operon containing a catalytically inactive nitrate reductase. The distinct transcriptional changes, proportional to the effect of these compounds on biofilm formation, include cyclic di-GMP (c-di-GMP) turnover genes. In support, ectopic expression of the c-di-GMP phosphodiesterase YhjH of Salmonella Typhimurium but not its catalytically inactive variant decreased biofilm formation. The respiration-dependent biofilm response of S. algae may permit differential colonization of environmental or host niches.
2020-12-05 | GSE133627 | GEO