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ABSTRACT: Screening of reference genes by qRT-PCR in Chelonus formosanus

PROVIDER: PRJNA1192507 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR31558521_1.fastq.gz	Fastqsanger.gz
	SRR31558521_2.fastq.gz	Fastqsanger.gz
	SRR31558522_1.fastq.gz	Fastqsanger.gz
	SRR31558522_2.fastq.gz	Fastqsanger.gz

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Screening of reference genes by qRT-PCR in Chelonus formosanus

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Project description:In this study, we investigated the transcriptome of Bifidobacterium bifidum PRL2010 during in vitro growth by micro array technology. When B. bifidum PRL2010 was grown in liquid broth, 425 of the 1644 PRL2010 genes represented on the array were expressed in at least one of the three investigated growth phases, i.e., lag-, exponential and stationary phase. These transcriptional analyses identified a core in vitro transcriptome encompassing 150 genes, which resulted expressed in all phases. A proportion of the latter genes were further investigated as potential reference genes by Quantitative Real Time PCR (qRT-PCR) assays. Their expression stability was evaluated under different growth conditions, encompassing cultivation on different carbon sources, exposure to environmental stresses (thermal, acidic and osmotic) and growth phases. Our analyses validated six reference genes suitable for normalizing mRNA expression levels in qRT-PCR experiments applied to bifidobacteria.

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