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The gene expression profile of primary BMMs differentiating into osteoclasts after knockout of solute carrier family 11 member 2 (SLC11A2)

ABSTRACT: The gene expression profile of primary BMMs differentiating into osteoclasts after knockout of solute carrier family 11 member 2 (SLC11A2)

PROVIDER: PRJNA1194049 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31602632_1.fastq.gz	Fastqsanger.gz
	SRR31602632_2.fastq.gz	Fastqsanger.gz
	SRR31602633_1.fastq.gz	Fastqsanger.gz
	SRR31602633_2.fastq.gz	Fastqsanger.gz
	SRR31602634_1.fastq.gz	Fastqsanger.gz

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The gene expression profile of primary BMMs differentiating into osteoclasts after knockout of solute carrier family 11 member 2 (SLC11A2)

Project description:To investigate the effect of solute carrier family 11 member 2 (SLC11A2) knockout on osteoclast differentiation, BMMs were extracted from the femurs of SLC11A2 knockout mice and the same litter of control mice, treated with RANKL, and then rna sequencing was performed.

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Project description:Long noncoding RNAs (lncRNAs) have emerged as integral regulators of physiology and disease, but specific roles of lncRNAs in bone resorption remain largely unknown. Here, RNA-seq analysis indicated that lncRNA Nron was a candidate funtional regulators of bone resorption. Our further experimental analysis showed that Nron regulates bone resorption in mice. We further identified a functional motif of Nron. Consequently, delivery of Nron functional motif specifically in osteoclasts reversed bone loss without obvious side-effects in vivo. Together, our study indicate that Nron is a key bone resorption suppressor and reveal Nron functional motif might be a potential therapeutic strategy to combat excessive bone absorption in osteoporosis.

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