Project description:We report raw bulk RNA sequencing data rice roots (X.kitaake) protoplasted for 2.5 hours and 3 hours to eliminate the effects of protoplasting duration on our scRNA-seq analysis, as well as rice roots grown in gel, non-compacted soil and compacted soil conditions to verify our findsing with scRNA-seq studies

2024-12-08 | GSE283428 | GEO

IntAct- IFA to prevent anastomotic leak in rectal cancer surgery

Project description:See https://www.cancerresearchuk.org/about-cancer/find-a-clinical-trial/a-study-looking-to-reduce-a-serious-side-effect-of-rectal-cancer-surgery-intact

| 2410037 | ecrin-mdr-crc

Non-intact membrane DNBR

Project description:Non-intact membrane DNBR Raw sequence reads

| PRJNA954296 | ENA

Gene Expression Profile Is Different between Intact and Enzymatically Digested Equine Articular Cartilage.

Project description:ObjectivesRNA isolation is necessary for the evaluation of gene expression. Due to the nature of its extracellular matrix, RNA isolation from articular hyaline cartilage is difficult and thus the tissue is commonly enzymatically digested in order to extract RNA from the obtained chondrocytes. We hypothesized that the digestion process affects the expression levels of common cartilage-associated genes.DesignExpression of cartilage-associated genes was compared between intact cartilage and digested chondrocytes from weight bearing and non-weight bearing regions of the equine fetlock joint.ResultsThe gene expression of SOX9, COL1A2, COL2A1, ACAN, and COLX were analyzed. Digested cartilage showed a significant decrease in the expression of COL1A2, COL2A1, and ACAN compared to intact cartilage in both joint regions, and an increase in COLX expression in non-weight bearing cartilage only.ConclusionsEnzymatic digestion of cartilage significantly impacts gene expression profile. We conclude that while RNA isolation from intact cartilage is more technically difficult, determination of gene expression should be conducted on intact cartilage if true representation of the in vivo processes is sought.

| S-EPMC7970368 | biostudies-literature

bulk RNA-seq of germinating Arabidopsis thaliana seeds with and without protoplasting

Project description:This experiment aims to determine the genes that respond to protoplasting treatment in a time course of Arabidopsis seed germination, so that they can be filtered out from single-cell RNA-seq analysis which involves protoplasting.

2023-01-11 | E-MTAB-12521 | biostudies-arrayexpress

RNA-Seq analysis of human osteoarthritic intact cartilage following total knee replacement

Project description:RNA-Seq analysis of human osteoarthritic intact cartilage following total knee replacement

| PRJEB23730 | ENA