Project description:6-year-old ramets of U. minor (Atinian elm clone) were inoculated with O. novo-ulmi in order to evaluate molecular responses activated during plant colonization. To elucidate the different genes involved in the U. minor immune system and the molecular changes suffered after inoculation, oligomicroarrays were constructed using the data from the transcriptome available in the Dryad database (Perdiguero et al., 2015; http://dx.doi.org/10.5061/dryad.ps837), and hybridized with cDNA obtained from the ramets over a time course following inoculation. Three biological replicates for control and inoculated plants for each sampling point (1, 3, 7, 14 and 21 days post-inoculation) were hybridised using two colors (inoculated vs control).

2017-12-31 | E-MTAB-5654 | biostudies-arrayexpress

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