Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Reduced TBX5 dosage causes human atrial disease by undermining developmental gene regulation of atrial cardiomyocyte identity [scRNA-seq]

ABSTRACT: Reduced TBX5 dosage causes human atrial disease by undermining developmental gene regulation of atrial cardiomyocyte identity [scRNA-seq]

PROVIDER: PRJNA1201585 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR31801433_1.fastq.gz	Fastqsanger.gz
	SRR31801433_2.fastq.gz	Fastqsanger.gz
	SRR31801434.fastq.gz	Fastqsanger.gz
	SRR31801435_1.fastq.gz	Fastqsanger.gz
	SRR31801435_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 15

Similar Datasets

Reduced TBX5 dosage causes human atrial disease by undermining developmental gene regulation of atrial cardiomyocyte identity [scRNA-seq]

Project description:In a TBX5-linked human cellular model of atrial disease, we examined the gene networks controlled by TBX5 during human atrial differentiation using single-cell RNA and ATAC sequencing.

2025-02-18 | GSE285169 | GEO

FOG2 modulates a TBX5/GATA4-co-ocupied atrial gene regulatory network linking heart failure to atrial fibrillation risk [RNA-Seq]

Project description:The transcription factor FOG2 (ZFPM2) is upregulated in human heart failure and increased FOG2 expression causes heart failure in mice. We found that FOG2 directly intersects a gene regulatory network driven by the atrial-enriched TF TBX5 and required for atrial cardiomyocyte rhythm control gene expression

2024-05-02 | GSE198734 | GEO

FOG2 modulates a TBX5/GATA4-co-ocupied atrial gene regulatory network linking heart failure to atrial fibrillation risk [ChIP-Seq]

2024-05-02 | GSE198733 | GEO

FOG2 modulates a TBX5/GATA4-co-ocupied atrial gene regulatory network linking heart failure to atrial fibrillation risk [ncRNA-Seq]

2024-05-02 | GSE198786 | GEO

Tbx5 regulatory element controls atrial function and heart size II

Project description:Left atrial RNA-seq of Tbx5(REint), Tbx5(REdown), Tbx5(Reint);Prrx1(RE) and control adult mice

2023-02-10 | GSE189342 | GEO

TBX5 KO multiome

Project description:Understanding how the atrial and ventricular chambers of the heart maintain their distinct identity is a prerequisite for treating chamber-specific diseases. Here, we selectively inactivated the transcription factor Tbx5 in the atrial working myocardium of the neonatal mouse heart to show that it is required to maintain atrial identity. Atrial Tbx5 inactivation downregulated highly chamber specific genes such as Myl7 and Nppa, and increased expression of ventricular identity genes including Myl2. Using combined single nucleus transcriptome and open chromatin profiling, we assessed genomic accessibility changes underlying the altered atrial identity expression program, identifying 1846 genomic loci with greater accessibility in control atrial cardiomyocytes compared to KO aCMs. 69% of the control-enriched ATAC regions were bound by TBX5, demonstrating a role for TBX5 in maintaining genomic accessibility. These regions were associated with genes that had higher expression in control aCMs compared to KO aCMs, suggesting they act as TBX5-dependent enhancers. To confirm this hypothesis we analyzed chromatin looping of enhancers marked by H3K27Ac using HiChIP and found 510 chromatin loops that were sensitive to TBX5 dosage. Of the loops enriched in control aCMs, 73.7% contained anchors in control-enriched ATAC regions. Conversely, Tbx5 overexpression in the ventricular myocardium drove atrial gene expression. Together, these data demonstrate a role for TBX5 in maintaining the atrial gene expression program by binding to atrial enhancers to preserve tissue-specific chromatin architecture. We highlight this phenomenon at major atrial identity genes including Nppa, Bmp10 and Myl7.

2023-08-11 | GSE222373 | GEO

TBX5 Adult Knockout Left Atrial Gene Expression

Project description:TBX5 removed from adult mice with R26CreERt2, left and right atrial RNA extracted and sequenced.

2019-04-09 | GSE129503 | GEO

TBX5 KO HiChIP

2023-08-11 | GSE222370 | GEO

TBX5 Adult Knockout Right Atrial Gene Expression [RA_RNAseq]

Project description:TBX5 removed from adult mice with R26CreERt2, right atrial RNA extracted and sequenced.

2023-08-02 | GSE239809 | GEO

TBX5 overexpression in ventricle

2023-08-11 | GSE222579 | GEO