Project description:We leverage RNA sequencing to identify the transcriptional changes assocaited with a TGFB1-induced epithelial-mesenchymal transition in the mouse ovarian surface epithelium
Project description:Adult Ovarian Surface Epithelium (OSE) retains the ability to undergo Epithelial to Mesenchymal Transition (EMT). We established a cell culture of murine adult OSE in their epithelial state, and we induced EMT by modifying their culture conditions. In this experiment we compare the transcriptional profile of the OSE before and after EMT.
Project description:Adult Ovarian Surface Epithelium (OSE) retains the ability to undergo Epithelial to Mesenchymal Transition (EMT). We established a cell culture of murine adult OSE in their epithelial state, and we induced EMT by modifying their culture conditions. In this experiment we compare the transcriptional profile of the OSE before and after EMT. Experiment Overall Design: Each of three different cultures of mouse OSE in their epithelial phenotype is split in two: one half is kept epithelial, the other half is cultured under conditions that induce the cells to undergo EMT. After one week the EMT process is complete (as assessed morphologically and biochemically) and the transcriptional profiles of the cells in their respective phenotypes are compared.
Project description:A cell line representative of human high-grade serous ovarian cancer (HGSOC) should not only resemble its tumor of origin at the molecular level, but also demonstrate functional utility in pre-clinical investigations. Here we report the integrated proteomic analysis of 26 ovarian cancer cell lines, HGSOC tumors, immortalized ovarian surface epithelial cells, and fallopian tube epithelial cells via a single-run mass spectrometric workflow. The in-depth quantitation of > 10,000 proteins results in three distinct cell line categories: epithelial (group I), clear cell (group II), and mesenchymal (group III). We identify a 67-protein cell line signature, which separates our entire proteomic dataset, as well as a confirmatory publicly available CPTAC/TCGA tumor proteome dataset, into a predominantly epithelial and mesenchymal HGSOC tumor cluster. This proteomics-based epithelial/mesenchymal stratification of cell lines and human tumors indicates a possible origin of HGSOC either from the fallopian tube or from the ovarian surface epithelium.
Project description:A cell line representative of human high-grade serous ovarian cancer (HGSOC) should not only resemble its tumor of origin at the molecular level, but also demonstrate functional utility in pre-clinical investigations. Here we report the integrated proteomic analysis of 26 ovarian cancer cell lines, HGSOC tumors, immortalized ovarian surface epithelial cells, and fallopian tube epithelial cells via a single-run mass spectrometric workflow. The in-depth quantitation of > 10,000 proteins results in three distinct cell line categories: epithelial (group I), clear cell (group II), and mesenchymal (group III). We identify a 67-protein cell line signature, which separates our entire proteomic dataset, as well as a confirmatory publicly available CPTAC/TCGA tumor proteome dataset, into a predominantly epithelial and mesenchymal HGSOC tumor cluster. This proteomics-based epithelial/mesenchymal stratification of cell lines and human tumors indicates a possible origin of HGSOC either from the fallopian tube or from the ovarian surface epithelium.
