ABSTRACT: Effect of dietary biofloc from a novel bacteriaponics recirculating aquaculture system on gut microbial community of barramundi (Lates calcarifer)
Project description:In this study, we attempt to characterize the transcriptomic profile of the Asian seabass gonads at various developmental stages. The protandric Asian seabass or barramundi (Lates calcarifer) typically matures as a male at approximately 2–4 years of age and then changes sex to a female in later years. For this experiment, Asian seabass of several ages were collected from the Marine Aquaculture Center of the Agri-Food & Veterinary Authority of Singapore and from farms around Singapore. There were no treatments carried out in this experiment. The gonads were examined by histology and classified according to sexual maturation status as described by Guiguen and colleagues (Guiguen et al. Environmental Biology of Fishes, 1994). Altogether, we analyzed 22 gonadal samples that could be classified into six different types of gonads.
Project description:In this study, we attempt to characterize the transcriptomic profile of the Asian seabass gonads at various developmental stages. The protandric Asian seabass or barramundi (Lates calcarifer) typically matures as a male at approximately 2M-bM-^@M-^S4 years of age and then changes sex to a female in later years. For this experiment, Asian seabass of several ages were collected from the Marine Aquaculture Center of the Agri-Food & Veterinary Authority of Singapore and from farms around Singapore. There were no treatments carried out in this experiment. The gonads were examined by histology and classified according to sexual maturation status as described by Guiguen and colleagues (Guiguen et al. Environmental Biology of Fishes, 1994). Altogether, we analyzed 22 gonadal samples that could be classified into six different types of gonads. Total 22 samples: Adult Ovaries (F3-stage; 5 years old fish) : 4 Adult Testes (M3-stage; 5 years old fish) : 4 Early Testes (M3-stage; 8-9 months old fish) : 3 Early Transforming Gonads (>2 years old fish) : 3 Late Transforming Gonads (>2 years old fish) : 4 Undifferentiated Gonads (4.5 months old fish) : 4
Project description:Cryptocaryon irritans is the causative agent of white spot disease that infects virtually all marine teleost including Lates calcarifer. We undertook to gain an informative snapshot of the host’s transcriptomic response during the course of infection. Liver and spleen cDNA libraries-based L. calcarifer cDNA microarray were used as a tool to oversee the infection response. Parasites infection was carried out on juvenile fish for four days, and total RNA was extracted from the daily harvested infected liver. cDNAs synthesized from respective daily pooled total RNA were labelled and hybridized onto the L. calcarifer chip. The majority of infection-induced genes are acute phase proteins (APP) with hepcidin, C-type lectin and serum amyloid A being the most up-regulated products.
