Project description:The presence of DNA methylation in beetles (Coleoptera) has only been investigated with bisulfite sequencing of Tribolium castaneum, which produced no evidence of DNA methylation. Here, we used whole genome bisulfite sequencing to assay if DNA methylation was present in another beetle, Nicrophorus vespilloides. We used T. castaneum as a negative control.
Project description:This series examines gene expression in the anterior midgut at several time points (2, 4, 8, & 16 h) after topical application of juvenile hormone III (JHIII) or acetone (control) to adult beetles of both sexes. Keywords = North American pine engraver beetle Keywords = anterior midgut Keywords = juvenile hormone Keywords = pheromone biosynthesis Keywords = Coleoptera Keywords = Scolytidae Keywords: other
Project description:This series examines gene expression in the anterior midgut at several time points (2, 4, 8, & 16 h for males, 8 h for females) after topical application of juvenile hormone III (JHIII) or acetone (control) to adult beetles. In addition, gene expression in male anterior midguts were examined 24 h after phloem feeding or in unfed beetles. Keywords: North American pine engraver beetle; anterior midgut; juvenile hormone; pheromone biosynthesis; Coleoptera; Scolytidae Publication reference: Reference Type: Book Section Authors: Tittiger, Claus; Keeling, Christopher I.; Blomquist, Gary J. Year: 2005 Title: Some insights into the remarkable metabolism of the bark beetle midgut. Editor: Romeo, J.T. Book Title: Chemical ecology and phytochemistry of forest ecosystems City: Toronto Publisher: Elsevier Volume: 39 Pages: 57-78 Series Title: Recent Advances in Phytochemistry Keywords: other
Project description:The Colorado potato beetle (CPB) is a major pest of potato crops that has evolved resistance to more than 50 pesticides. For decades, CPB has been a model species for research on insecticide resistance, insect physiology, diapause, reproduction and evolution. Yet, the research progress in CPB is constrained by the lack of comprehensive genomic and transcriptomic information. Here, building on a recently established chromosome-level genome assembly, we built a gene expression atlas of the CPB using the transcriptomes of 61 samples representing major organs and developmental stages. By using both short and long reads, we improved the genome annotation and identified 6,623 more genes that were missed in previous annotations. We then established a web portal allowing the search and visualization of the gene expression atlas for the research community. The CPB atlas provides useful tools and comprehensive gene expression data, which will accelerate future research in both pest control and insect biology fields.
Project description:Bark beetles (Coleoptera: Scolytinae) are pests of many forests around the world. The mountain pine beetle (MPB), Dendroctonus ponderosae Hopkins, is a significant pest of western North American pine forests. The MPB is able to overcome the defences of pine trees through pheromone-assisted aggregation that results in a mass attack of host trees. These pheromones, both male and female produced, are believed to be biosynthesized in the midgut and/or fat body of these insects. We have used transcriptomics (RNA-seq) to identify transcripts differentially expressed between sexes and between tissues, with juvenile hormone III treatment, which is known to induce pheromone biosynthesis.