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Symmetric DNA Adenine Methylation Plays an Essential Role in Early-Diverging Fungi [RNA-seq]

ABSTRACT: Symmetric DNA Adenine Methylation Plays an Essential Role in Early-Diverging Fungi [RNA-seq]

PROVIDER: PRJNA1203249 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31834732_1.fastq.gz	Fastqsanger.gz
	SRR31834732_2.fastq.gz	Fastqsanger.gz
	SRR31834733_1.fastq.gz	Fastqsanger.gz
	SRR31834733_2.fastq.gz	Fastqsanger.gz
	SRR31834734_1.fastq.gz	Fastqsanger.gz

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Symmetric DNA Adenine Methylation Plays an Essential Role in Early-Diverging Fungi

Project description:Symmetric DNA Adenine Methylation Plays an Essential Role in Early-Diverging Fungi

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Symmetric DNA Adenine Methylation Plays an Essential Role in Early-Diverging Fungi

Project description:The discovery of N6-methyladenine (6mA), typically found in prokaryotic DNA, in eukaryotic genomes, has revolutionized epigenetics. Here, we show that symmetric 6mA is essential in the early diverging fungus Rhizopus microsporus, as the absence of the MT-A70 complex (MTA1c) responsible for this modification results in a lethal phenotype. 6mA is present in 70% of the genes, correlating with the presence of H3K4me3 and H2A.Z in open euchromatic regions. This modification is found predominantly in nucleosome linker regions, influencing the nucleosome positioning around the transcription start sites of highly expressed genes. Controlled downregulation of MTA1c results in a reduction of symmetric 6mA sites affecting nucleosome positioning and histone modifications, leading to altered gene expression, which is likely the cause of lethality. Our study highlights the indispensable role of the DNA 6mA in a multicellular organism and delineates the mechanisms through which this epigenetic mark regulates gene expression in a eukaryotic genome

2025-01-12 | GSE276939 | GEO

Symmetric DNA Adenine Methylation Plays an Essential Role in Early-Diverging Fungi [ChIP-seq]

Project description:Symmetric DNA Adenine Methylation Plays an Essential Role in Early-Diverging Fungi [ChIP-seq]

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Symmetric DNA Adenine Methylation Plays an Essential Role in Early-Diverging Fungi [ChIP-seq]

2025-01-12 | GSE276938 | GEO

Symmetric DNA Adenine Methylation Plays an Essential Role in Early-Diverging Fungi [RNA-seq]

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Earliest Diverging Fungi and Rotosphaerida in Beijing

Project description:Earliest Diverging Fungi and Rotosphaerida in China Raw sequence reads

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AC-motif: A DNA motif containing adenine and cytosine repeat plays a role in gene regulation

Project description:AC-motif: A DNA motif containing adenine and cytosine repeat plays a role in gene regulation

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Adenine effect on Enterohemorraghic E.coli

Project description:Previous experiments have shown that E. feacalis increases EHEC virulence by secreting adenine, this RNAseq aims to understand the molecular mechanism underlaying adenine role on EHEC

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Chytrid fungi

Project description:Mycoviruses in Early-Diverging Fungi

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RNA methylation maintains hematopoietic stem cell identity and symmetric commitment

Project description:Stem cells balance cellular fates through asymmetric and symmetric divisions in order to self-renew or to generate downstream progenitors. Symmetric commitment divisions in stem cells are required for rapid regeneration during tissue damage and stress. The control of symmetric commitment remains poorly defined. N6-methyladenosine (m6A), the most abundant posttranscriptional mRNA modification controls cellular states and its abundance is dysregulated in cancer. Here we show that mRNA methylation controls symmetric commitment and cell identity of hematopoietic stem cells (HSCs). Using single-cell RNA sequencing (scRNA-seq) in combination with transcriptomic profiling of HSPCs (hematopoietic stem and progenitor cells) from control and m6A methyltransferase Mettl3 conditional knockout mice, we found that m6A-deficient HSC fail to symmetrically differentiate. Dividing HSCs are expanded and are blocked in an intermediate state that molecularly and functionally resembles multipotent progenitors. Mechanistically, RNA methylation controls Myc mRNA abundance in differentiating HSCs. Importantly, we identified MYC as a new marker for HSC asymmetric and symmetric commitment. Furthermore, forced expression of MYC rescued m6A’s requirement for engraftment indicating its importance in the early stage of HSC cellular fate. Overall our results indicate that RNA methylation is critical for normal blood homeostasis and may provide a general mechanism for how stem cells regulate differentiation fate choice.

2019-07-25 | GSE132357 | GEO

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