Project description:In order to understand the molecular mechanisms of salinity adaptation, high-throughput RNA-Seq was utilized to discover the gene expression profiles and pathways that responded to elevated salinity in the liver of T. s. elegans
2018-11-16 | GSE117354 | GEO
Project description:salinity-contained anaerobic system (archaea)
| PRJNA740143 | ENA
Project description:salinity-contained anaerobic system (bacteria)
Project description:Anaerobic digestion is a popular and effective microbial process for waste treatment. The performance of anaerobic digestion processes is contingent on the balance of the microbial food web in utilizing various substrates. Recently, co-digestion, i.e., supplementing the primary substrate with an organic-rich co-substrate has been exploited to improve waste treatment efficiency. Yet the potential effects of elevated organic loading on microbial functional gene community remains elusive. In this study, functional gene array (GeoChip 5.0) was used to assess the response of microbial community to the addition of poultry waste in anaerobic digesters treating dairy manure. Consistent with 16S rRNA gene sequences data, GeoChip data showed that microbial community compositions were significantly shifted in favor of copiotrophic populations by co-digestion, as taxa with higher rRNA gene copy number such as Bacilli were enriched. The acetoclastic methanogen Methanosarcina was also enriched, while Methanosaeta was unaltered but more abundant than Methanosarcina throughout the study period. The microbial functional diversity involved in anaerobic digestion were also increased under co-digestion.
Project description:Prochlorococcus is an obligate marine microorganism which are dominant autotroph in tropical and subtropical central oceans. However, what is the low salinity boundary and how Prochlorococcus would response to low salinity exposure is still unknown. In this study, we first tested the growing salinity range of two Prochlorococcus strains, NATL1A and MED4, and then compared the global transcriptome of their low salinity acclimated cells and cells growing in normal seawater salinity. We found that MED4 could be acclimated in the lowest salinity of 25% and NATL1A could be acclimated in the lowest salinity of 28%. Measurement of the effective quantum yield of PSII photochemistry (Fv/Fm) indicated that both strains were stressed when growing in salinity lower than 34%. The transcriptomic response of NATL1A and MED4 were approximately different, with much more genes having changed transcript abundance in NATL1A than in MED4. To cope with low salinity, NATL1A downregulated the transcript of most genes involved in translation, ribosomal structure and biogenesis, while MED4 upregulated those genes. Moreover, low salinity acclimated NATL1A cells suppressed ATP-producing genes and induced the expression of photosynthesis related genes, while low salinity acclimated MED4 upregulated ATP-producing genes and downregulated photosynthesis related genes. These results indicate that the response to low salinity stress of different Prochlorococcus strains could be distinct. The study provided the first glimpse into the growing salinity range of Prochlorococcus cells and their global gene expression changes due to low salinity stress.
Project description:The emerging foodborne pathogen, Aeromonas hydrophila, co-infects humans and animals, especially fish, threatening aquacultural production and public health. Previously we found that Scatophagus argus, a widely cultivated fish species with high economic value, exhibited enhanced growth but increased susceptibility to A. hydrophila infection under freshwater conditions compared to seawater conditions. However, the exact mechanisms involved remain unclear.Our study demonstrated that enhanced virulence of A. hydrophila 201416 isolated from S. argus induced by increasing salinity was associated with altered quorum sensing-related gene expression and regulated behaviors. Results from virulence assays combining phenotypic characterization indicated that increased salinity (from 0 to 35 g/L NaCl) impeded Ah201416 infection of S. argus, a trend aligning with increased biofilm mass and swimming motility, but opposite to bacterial growth. RNA-sequencing and quantitative reverse transcriptional PCR analysis confirmed significant upregulation of genes related to flagellar assembly (flgB, flgH, flgC, flgI, flhA, and fliA), bacterial secretion (HlyD and Ahh1), and quorum sensing (AhyR, LuxO, and LuxE) of Ah201416 in response to elevated salinity. These findings suggested that increased salinity not merely enhanced the virulence of Ah201416 but bolstered the resistance of S. argus, thereby mitigating its susceptibility. This study provides further insights into the microbial risks associated with A. hydrophila in aquacultural production, which is critical to developing appropriate prevention and control strategies and ensuring safe seafood supply.