Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA120535 | ENA |
REPOSITORIES: ENA
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Project description:The mammalian liver possesses a remarkable regenerative ability. 1) The 'oval cell' response emanates from the biliary tree when all hepatocytes are affected by chronic liver disease. 2) A massive, proliferative response of mature hepatocytes occurs upon acute liver damage such as partial hepatectomy (PHx). We establish a long-term 3D organoid culture system from mouse and human fetal/pediatric/adult hepatocytes that retain key morphological and functional features of hepatocytes fate. We report the mRNA and single cell sequencing of Hep-Orgs from different donors in different passages. We compared Hep-Orgs with primary hepatocytes, proliferative hepatocytes or Chol-Orgs derived from Epcam+ biliary cells. By analyzing, we determine similar gene expression profile of Hep-Orgs with primary hepatocytes and make genes lists distinguished with undamaged hepatocytes as well. We find the Hep-Orgs resemble proliferative damage-response of hepatocytes after partial hepatectomy while Chol-Orgs express high cholangiocytes markers. The sequencing data constitutes a valuable resource to understand liver organoids especially Hep-Orgs.
2018-11-29 | GSE111301 | GEO
Project description:Germline cell-derived pluripotent stem cells (GPSCs) are similar to embryonic stem (ES) cells in that they can proliferate intensively and differentiate into a variety of cell types, including cardiomyocytes and neurons. In this report, mouse GPSCs were induced to differentiate into hepatocytes with very high efficiency, and demonstrated, for the first time, to be functional. These hepatocytes were characterised at cellular and molecular levels. The GPSC-derived hepatocytes not only expressed hepatic markers, but were also metabolically active as shown by albumin and haptoglobin secretion, urea synthesis, glycogen storage and indocyanine green uptake. Previous studies have revealed some inherent differences in gene expression between undifferentiated mouse ES cells and GPSCs. We wanted to investigate whether this difference may impact on the hepatocyte differentiation capacity of the GPSCs. Large-scale gene expression profiling revealed a strong similarity between GPSC and ES cells at different stages of induced hepatic differentiation. Moreover, Pearson correlation analysis of the microarray datasets revealed that, at late hepatic differentiation stages, the in vitro-derived cells were closer to fetal mouse primary hepatocytes. Thus, adult GPSCs offer great potential for cell ment therapy for a wide variety of liver diseases. Mouse ES cells and GPSCs at various times of hepatocyte differentiation, compared to embryonal (E16) and postnatal (PN1) mouse primary hepatocytes. The supplementary file 'GSE19044_non-normalized.txt' contains non-normalized data for Samples GSM471318-GSM471359.
2010-06-08 | E-GEOD-19044 | biostudies-arrayexpress
Project description:Comparison between mouse ES/iPS derived neurosphere and mouse primary culture of neurospheres obtained from fetal mouse ganglionic eminence
| PRJNA145353 | ENA
Project description:Microarrays were used to examine gene expression differences between primary human hepatocytes and hESC derived hepatoblast cultures treated or non-treated with cAMP. hESC's were differentiated with a hepatic cell lineage specific protocol that included the use of a 3-dimensional cell aggregation culturing technique. Followed by cAMP signaling, this promoted the maturation of hepatoblasts into a more hepatocyte-like population. Importantly, key enzymes related to liver function show that cAMP induced populations have a gene expression profile that is similar to primary human hepatocytes. Total RNA obtained from cultured primary hepatocytes and hESC derived hepatic populations.
2013-07-04 | E-GEOD-39157 | biostudies-arrayexpress
Project description:Expression data from primary hepatocytes from mouse
| PRJNA391465 | ENA
Project description:Introduction: Hepatic cell transplantation offers an alternative to orthotopic liver transplantation for chronic liver disease. We previously demonstrated that fetal rat hepatocytes durably persist and proliferate when transplanted to an injured adult liver. To identify mechanisms underlying fetal hepatocyte repopulation, we profiled gene expression and histone post-translational modifications (hPTM) of post-transplantation fetal colonies and surrounding liver, as well as those of primary fetal and adult hepatocytes. Methods: Using the DPPIV rat model, we transplanted and traced fetal hepatocytes into adult hosts. At 10 months after transplantation, we used laser capture microscopy to isolate fetal-derived colonies and surrounding adult host tissue. RNA and histones were extracted from laser captured tissue and isolated hepatocytes for RNA-seq and quantitation of hPTM. Results: Principal component analysis of RNA-seq results discriminated between fetal-derived colonies and surrounding adult host tissue. We identified 953 differentially expressed genes, many of which were significantly overexpressed in pre-transplant fetal hepatocytes relative to adult hepatocytes. This gene set included a disproportionate number of genes encoding ion transmembrane transporters. Proteomic analyses identified 13 distinct marks on Histone H3 whose relative abundance differed significantly between fetal-derived colonies and adult host tissue. Of these 13 marks, 11 had abundance profiles similar to those of fetal hepatocytes. Conclusions: A distinct gene expression and epigenetic profile seen in pre-transplant fetal cells is retained for at least 10 months following transplantation. Ontological and pathway analysis indicates activation of ion transporters, which may relate to the growth advantage of transplanted fetal cells.
2023-04-27 | GSE201757 | GEO
Project description:Expression Profiles of Primary Mouse Hepatocytes treated with Cyclosporin A and solvent control [miRNA]
| PRJNA241196 | ENA
Project description:Expression Profiles of Primary Mouse Hepatocytes treated with Cyclosporin A and solvent control [RNA]
| PRJNA241195 | ENA
Project description:To investigate the effect of TRIB3 overexpression on regulation of lipid metabolism in hepatocytes, we isolated mouse primary hepatocytes from AAV-GFP or AAV-Trib3 mice. We then performed gene expression profiling analysis using data obtained from RNA-seq of two groups of mouse primary hepatocytes from AAV-GFP or AAV-Trib3 mice.
2023-05-28 | GSE232807 | GEO