Project description:This study aims to provide referrable transcriptomic data for the exploration of floral pigment biosynthesis in hyacinth cultivars. This dataset consists of 189 RNA-Seq libraries prepared for seven cultivars at different developmental stages and perianth partitions, generated 1256.8 gigabytes data in total. After removal of adaptor trimming and quality trimming, the qualified sequences were adopted for de novo assembly of mRNA. The gene expressions were quantified as read counts and then normalized into FPKM. This study provides the first profiling on the gene expression of hyacinth, contributing to the new-generation molecular breeding in producing noval hyacinth cultivars.
2025-01-12 | GSE286406 | GEO
Project description:Microbial diversity analysis of water hyacinth silage
| PRJNA892783 | ENA
Project description:The complete chloroplast genome of Asian water hyacinth (Eichhornia crassipes)
Project description:Transcriptome sequencing (RNA-seq) was used to profile genome-wide transcript abundance in the primary root growth zone (PRGZ) of maize seedlings grown in different water deficit treatments: well-watered (-0.02 MPa), mild water deficit stress (-0.3 MPa), or severe water deficit stress (-1.6 MPa). For each water deficit treatment, the PRGZ transcriptome was profiled at 26 hours after initiation of the water deficit treatment. By comparing the abundance of each transcript under mild or severe water deficit stress relative to its abundance under well-watered conditions, we identified transcripts that are differentially regulated in the PRGZ in response to the two levels of water deficit stress.
Project description:Transcriptome approach of small RNA was performed at two stages of seed development during early maturation (24DAP) and seed maturity (42DAP) to differentially profile smallRNA accumulation from Xcf-inoculated and water-inoculated seeds.
Project description:Background: MicroRNAs are endogenous small noncoding RNAs that play critical roles in plant abiotic stress responses. The interaction between miRNA-mRNA targets and their regulatory pathways in response to water deficit stress has been investigated in many plant species. However, the miRNA transcriptome of durum wheat (Triticum turgidum L. ssp. durum) is poorly characterised, with little known about miRNA functions related to water deficit stress. Yield loss in durum wheat can be exacerbated due to minimal rainfall in the early reproductive stages of development during Spring in Australia. This study describes genotypic differences in the miRNAome between water deficit tolerant/sensitive durum, using flag leaf and developing head tissue, and more specifically identifies miRNAs associated with water deficit stress. Results: Small RNA libraries (96 in total) were constructed from flag leaf and developing head tissues of four durum genotypes (Tamaroi, Yawa, EGA Bellaroi, Tjilkuri), with or without water deficit stress. Illumina sequencing and subsequent analysis detected 110 conserved miRNAs and 159 novel candidate miRNA hairpins. Statistical analysis of the abundance of sequencing reads revealed 66 conserved miRNAs and five novel miRNA hairpins showing differential expression under water deficit stress. During stress, several conserved and novel miRNAs showed unambiguous inverted regulatory profiles between the durum genotypes studied. Several miRNAs were also identified to have different abundance in the flag leaf compared to the developing head regardless of treatment. Predicted mRNA targets from four novel durum miRNAs were characterised using Gene Ontology (GO) which revealed functions common to stress responses and plant development. Conclusion: For the first time, we present a comprehensive study of the miRNA transcriptome of flag leaf and developing head tissues in different durum genotypes under water deficit stress. The identification of differentially expressed miRNAs provides molecular evidence that miRNAs are potential determinants of water stress tolerance in durum wheat. GO analysis of predicted targets contributes to the understanding of genotype-specific physiological responses leading to stress tolerance capacity. Further functional analysis of specific stress responsive miRNAs identified, and their interaction with mRNA targets is ongoing and will assist in developing future durum wheat varieties with enhanced water deficit stress tolerance.
2015-10-01 | GSE69339 | GEO
Project description:A reference genome of Commelinales provides insights into the commelinids evolution and global spread of water hyacinth ( Pontederia crassipes)
Project description:A reference genome of Commelinales provides insights into the commelinids evolution and global spread of water hyacinth (Eichhornia crassipes)