Project description:Examined expression of microRNAs in different life-cycle stages and gut tissue of the pathogenic nematode Haemonchus contortus and identified differentially expressed miRNAs. Custom array contained probes to 609 Haemonchus contortus miRNA sequence (all mature and start miRNAs identified by Winter et al., 2012, BMC Genomics13:4; DOI: 10.1186/1471-2164-13-4) and all C. elegans sequences in miRBase release 16
Project description:Illumina sequencing of small RNAs from Brugia pahangi and Haemonchus contortus 4 samples examined, larval stage 3 and mixed sex adults from two parasitic nematode species.
Project description:Once Haemonchus contortus infects sheep it receives a series of host attacks, especially those relating to the infected animal’s T lymphocytes immune response. To obtain a systematic genome-wide profiling of the T lymphocyte genes involved, microarrays were used to compare gene expression between 0 days post infection (dpi), 3-5 dpi, 25-30 dpi and 60dpi in infected sheep. In this 853, 242 and 42 differentially expressed genes were acquired in the 3d vs. 0d comparison, the 30d vs. 0d comparison and the 60d vs. 0d comparison, respectively. Gene Ontology and pathway analysis indicated that modulated genes including SUGT1, FCER1G, CD23, IL-13 and galectin-14, were mostly associated with cellular homeostasis maintaining and immune response. Haemonchus contortus infection induced gene expression in sheep T lymphocytes was measured at 0, 3, 30 and 60 days post infection. Four time-series experiments were performed at each sheep (2#, 3# and 5#).
