Project description:Transcriptional profiling of Arabidopsis rossette leaves comparing WT Col-0 with a transgenic line overexpressing Ah24 gene from Amaranthus hypochondriacus.
Project description:Transcriptional profiling of Arabidopsis rossette leaves comparing WT Col-0 with a transgenic line overexpressing AhDGR gene from Amaranthus hypochondriacus.
Project description:Amaranthus hypochondriacus is a highly nutritious alternative grain native to Central and South America but consumed worldwide. Increased interest in A. hypochondriacus has driven characterization of bioactive secondary metabolites and their impact on the human body. A. hypochondriacus seeds are known to contain bioactive small molecules but there is a dearth of knowledge regarding endogenous bioactive peptides, especially cysteine-rich peptides (CRPs) which may be resistant to human digestion. Here, 89 CRPs were predicted in silico from A. hypochondriacus, 13 of which were detected in a seed extract via bottom-up proteomics, providing direct evidence for the translation of snakins, hevein-like peptides, defensins, lipid transfer proteins, and α-hairpinins. Mature forms of four novel and two known CRPs were molecularly characterized via top-down mass spectrometry. Four peptides demonstrated resistance to in vitro gastrointestinal digestion, suggesting that A. hypochondriacus CRPs may exhibit bioactivity after consumption and should be prioritized for further characterization.
Project description:Transcriptional profiling of Arabidopsis rossette leaves comparing WT Col-0 with a transgenic lines overexpressing AhNF-YC gene from Amaranthus hypochondriacus in three different conditions.
Project description:Transcriptional profiling of Arabidopsis rossette leaves comparing WT Col-0 with a transgenic line overexpressing AhERF or AhDOF genes from Amaranthus hypochondriacus under different conditions.
Project description:Transcriptional profiling of Arabidopsis rossette leaves comparing WT Col-0 with a transgenic line overexpressing Ah24 gene from Amaranthus hypochondriacus. One-condition experiment WT vs Ah24 OE plant leaves.
Project description:Transcriptional profiling of Arabidopsis rossette leaves comparing WT Col-0 with a transgenic lines overexpressing AhNF-YC gene from Amaranthus hypochondriacus in three different conditions. Three-condition experiment WT vs AhNF-YC OE plant leaves. The analyzed conditions were: normal growth conditions, water stress for 5 days and 24 hrs of recovery after normal watering was reestablished to 8-day water stressed plants.
