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Inhibition of hypoxic exosomal miR-423-3p decreases glioma progression by restricting autophagy in astrocytes

ABSTRACT: Inhibition of hypoxic exosomal miR-423-3p decreases glioma progression by restricting autophagy in astrocytes

PROVIDER: PRJNA1209555 | ENA |

REPOSITORIES: ENA

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Inhibition of hypoxic exosomal miR-423-3p decreases glioma progression by restricting autophagy in astrocytes

Project description:The tumor microenvironment (TME) of gliomas comprises glioma cells and surrounding cells, such as astrocytes, macrophages, T cells, and neurons. In the TME, glioma cells can activate normal human astrocytes (NHAs) through the secretion of exosomes and the activation of astrocytes can further improve the progression of glioma, leading to a poor prognosis for patients. However, the molecular mechanisms underlying NHAs activation by gliomas remain largely unknown. It this study, glioma-derived exosomes (GDEs) play an important role in the modulation of autophagy and activation of NHAs. Compared with normoxic glioma-derived exosomes, hypoxic glioma-derived exosomes (H-GDEs) further improved autophagy and activation of astrocytes, which strongly promoted the progression of glioma cells. In an miRNA array between two types of exosomes from gliomas, miR-423-3p was highly expressed in H-GDEs and played an important role in autophagy, resulting in the activation of NHAs. The mechanism by which hypoxic glioma cells react with NHAs to create an immunosuppressive microenvironment was identified and 15d-PGJ2 was established as an effective inhibitor of miR-423-3p to suppress NHA activation. These findings provide new insights into the diagnosis and treatment of gliomas by targeting autophagy and miR-423-3p expression.

2025-01-17 | GSE286468 | GEO

Ceratobasidium sp. 423

Project description:Ceratobasidium sp. 423 Transcriptome - Cer423

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Effect of overexpression of miR-423-5p on gene expression of endothelial cells.

Project description:To investigate machanism of miR-423-5p regulating the angiogenic ability of bEnd.3 cells, we transfected miR-423-5p mimic to overexpress miR-423-5p in bEnd.3 cells. Then we performed high throughput sequencing of miR-423-5p mimic-transfected and control bEnd.3 cells to evaluate different gene expressions between miR-210-3p-overexpressing and control.

2024-06-14 | GSE262133 | GEO

Ceratobasidium sp. 423

Project description:Ceratobasidium sp. 423 Resequencing genome sequencing

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Brevibacillus thermoruber 423

Project description:Brevibacillus thermoruber 423 Genome sequencing and assembly

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Ceratobasidium sp. 423

Project description:Ceratobasidium sp. 423 Standard Draft genome sequencing

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Deficiency of lysophosphatidic acid receptor 3 decreases the erythropoietin production in hypoxic mouse kidney

Project description:Deficiency of lysophosphatidic acid receptor 3 decreases the erythropoietin production in hypoxic mouse kidney

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Populus trichocarpa strain:Besc-423

Project description:Populus trichocarpa strain:Besc-423 Genome sequencing

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Panicum virgatum cultivar:AP13xWBC3xDAC6xVS16 - 423

Project description:Panicum virgatum AP13xWBC3xDAC6xVS16 - 423 - Mapping Population genome sequencing

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The Identification of Plasma Exosomal miR-423-3p as a Potential Predictive Biomarker for Prostate Cancer Castration-Resistance Development by Plasma Exosomal miRNA Sequencing.

Project description:Castration-resistant prostate cancer (CRPC) is the major cause of death from prostate cancer. Biomarkers to improve early detection and prediction of CRPC especially using non-invasive liquid biopsies could improve outcomes. Therefore, we investigated the plasma exosomal miRNAs associated with CRPC and their potential for development into non-invasive early detection biomarkers for resistance to treatment. RNA-sequencing, which generated approximately five million reads per patient, was performed to identify differentially expressed plasma exosomal miRNAs in 24 treatment-naive prostate cancer and 24 CRPC patients. RT-qPCR was used to confirm the differential expressions of six exosomal miRNAs, miR-423-3p, miR-320a, miR-99a-5p, miR-320d, miR-320b, and miR-150-5p (p = 7.3 × 10-8, 0.0020, 0.018, 0.0028, 0.0013, and 0.0058, respectively) firstly in a validation cohort of 108 treatment-naive prostate cancer and 42 CRPC patients. The most significant differentially expressed miRNA, miR-423-3p, was shown to be associated with CRPC with area under the ROC curve (AUC) = 0.784. Combining miR-423-3p with prostate-specific antigen (PSA) enhanced the prediction of CRPC (AUC = 0.908). A separate research center validation with 30 treatment-naive and 30 CRPC patients also confirmed the differential expression of miR-423-3p (p = 0.016). Finally, plasma exosomal miR-423-3p expression in CRPC patients was compared to 36 non-CRPC patients under androgen depletion therapy, which showed significantly higher expression in CRPC than treated non-CRPC patients (p < 0.0001) with AUC = 0.879 to predict CRPC with no difference between treatment-naive and treated non-CRPC patients. Therefore, our findings demonstrate that a number of plasma exosomal miRNAs are associated with CRPC and miR-423-3p may serve as a biomarker for early detection/prediction of castration-resistance.

| S-EPMC7817948 | biostudies-literature

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