Project description:scRNA-seq was used in order to produce a cell type atlas of the larval and adult sea lamprey (Petromyzon marinus) brain. This resource enabled us to reveal the cell type composition and molecular organization of a representative of a lineage (i.e., the cyclostomes) that diverged from the rest of vertebrates around 500 million years ago, and lays the foundations for a better comprehension of vertebrate brain evolution
Project description:DNA was isolated from tissues (blood, sperm, or liver). DNA from different tissues from the same organism were labeled with alternate fluorophores (Cy3 or Cy5) and hybridized to a custom array that was designed from lamprey (Petromyzon marinus) genomic sequence.
Project description:Microarray analysis was used for a global investigation of the cellular effects of acute 3-trifluoromethyl-4-nitrophenol (TFM) exposure on Saccharomyces cerevisiae over time. TFM is used to control sea lamprey (Petromyzon marinus) populations in the Lake Champlain and Great Lakes regions. Little is known about the changes in gene expression due to TFM exposure so this time course microarray study was performed to reveal significantly altered patterns of gene expression when yeast cultures were exposed to 0.05mM TFM over four hours.
Project description:DNA was isolated from tissues (blood, sperm, or liver). DNA from different tissues from the same organism were labeled with alternate fluorophores (Cy3 or Cy5) and hybridized to a custom array that was designed from lamprey (Petromyzon marinus) genomic sequence. 1 animal - sperm vs. blood, 2 animals - blood vs. liver
