Project description:We investigated the molecular mechanisms for osteolytic bone metastasis by selecting human lung cancer cell line subpopulations with elevated metastatic activity and validating genes that are overexpressed in these cells. A bone-seeking squamous lung cancer cell line (HARA-B4) was established by sequentially injecting parental HARA cells into the left ventricle of male 5-week-old nude mice 4 times. The parental line HARA and highly bone-metastatic line HARA-B4 were analized. Total of two samples. No replicates.
Project description:We investigated the molecular mechanisms for osteolytic bone metastasis by selecting human lung cancer cell line subpopulations with elevated metastatic activity and validating genes that are overexpressed in these cells. A bone-seeking squamous lung cancer cell line (HARA-B4) was established by sequentially injecting parental HARA cells into the left ventricle of male 5-week-old nude mice 4 times.
Project description:The purpose of this study to examine the effect of Calbindin expression, driven by an upstream HERVH LTR (human endogenous retrovirus H long terminal repeat), in HARA squamous lung cancer cells. Parental HARA cells, Calbindin-deficient HARA 3D5 cells, HARA.LTR7-GFP+ and HARA.LTR7-GFP- cells were compared and were processed as a single batch, using the 10× Genomics Chromium platform.
