ABSTRACT: Sequencing and description of mitochondrial genome of Coquillettidia venezuelensis, Trichoprosopon digitatum, and Uranotaenia calosomata (Diptera: Culicidae)
Project description:In Sub-Saharan Africa, Anopheles gambiae Giles (Diptera: Culicidae) largely contributes to malaria transmission, in direct relation to environmental conditions influencing the vector ecology. Therefore, our study aimed to compare the proteomes of An. gambiae according to varying insecticide pressures associated to cotton crops also integrating different population origins from two climatic regions of Burkina Faso.
Project description:Penicillium digitatum is the pathogen of Green mold in Postharvest citrus. After inoculating Penicillium digitatum into the wound of citrus to infect it, transcriptome sequencing was carried out and compared with the results of transcriptome sequencing of Penicillium digitatum before inoculation in order to screen the differentially expressed genes and reveal its infection mechanism.
Project description:In spite of the many recent developments in the field of vector sialomics, the salivary glands of larvalmosquitoes have been largely unexplored. We used whole-transcriptome microarray analysis to create a gene-expression profile of the salivary gland tissue of fourth-instar Anopheles gambiae larvae, and compare it to the gene-expression profile of a matching group of whole larvae. We identified a total of 221 probes with expression values that were (a) significantly enriched in the salivary glands, and (b)sufficiently annotated as to allow the prediction of the presence/absence of signal peptides in their corresponding gene products. Based on available annotation of the protein sequences associated with these probes, we propose that the main roles of larval salivary secretions include: (a) immune response, (b) mouthpart lubrication, (c) nutrient metabolism, and (d) xenobiotic detoxification. Other highlights of the study include the cloning of a transcript encoding a previously unknown salivary defensin (AgDef5), the confirmation of mucus secretion by the larval salivary glands, and the first report of salivary lipocalins in the Culicidae. Keywords: Anopheles gambiae, salivary gland, Diptera, gene expression, salivary defensin, transcriptome, salivary lipocalin
Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
