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Effect PELO knockdown in FOCAD intact vs deleted KP4 pancreatic cancer cells

ABSTRACT: Effect PELO knockdown in FOCAD intact vs deleted KP4 pancreatic cancer cells

PROVIDER: PRJNA1213801 | ENA |

REPOSITORIES: ENA

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Pancreatic Cancer HPAF/CD18 cells: Ecd knockdown vs Scrambled

Project description:Purpose: To study the expression and function of a novel cell cycle regulatory protein, human ecdysoneless (Ecd), during pancreatic cancer (PC) pathogenesis. Experimental Design: Immunohistochemical expression profiling of Ecd was done in non-neoplastic normal pancreatic tissues and pancreatic ductal adenocarcinoma lesions (from tissue microarray and Rapid Autopsy program) as well as precancerous PanIN lesions and metastatic organs. To analyze the biological significance of Ecd in PC progression, Ecd was stably knocked down in PC cell line followed by in vitro and in vivo functional assays. Results: Normal pancreatic ducts show very weak to no Ecd expression compared to significant positive expression in PC tissues (mean±SE composite score: 0.3±0.2 and 3.8±0.2 respectively, p<0.0001) as well as in PanIN precursor lesions with a progressive increase in Ecd expression with increasing dysplasia (PanIN-1 to PanIN-3). Analysis of matched primary tumors and metastases from PC patients revealed that Ecd is highly expressed in both primary pancreatic tumor and in distant metastatic sites. Further, knockdown of Ecd suppressed cell proliferation in vitro and tumorigenicity of PC cells in mice orthotopic tumors. Microarray study revealed that Ecd regulates expression of glucose transporter GLUT4 in PC cells and was subsequently shown to modulate glucose uptake, lactate production and ATP generation by PC cells. Finally, knockdown of Ecd also reduced level of pAkt, key signaling molecule known to regulate aerobic glycolysis in cancer cells. Conclusion: Ecd is a novel tumor promoting factor that is differentially expressed in pancreatic cancer and potentially regulates glucose metabolism within cancer cells. Two-condition experiment, Ecd knockdown vs Scrambled cells. Biological replicates: 3 Ecd knockdownl, 3 Scrambled, independently grown and harvested. One replicate per array

2012-08-09 | E-GEOD-39988 | biostudies-arrayexpress

Effect PELO knockdown in FOCAD intact vs deleted KP4 pancreatic cancer cells

Project description:To invetigate how sensitive cells respond to PELO suppression we performed a bulk RNA-seq in an isogenic pair of KP4 cell line with or without FOCAD deletion.

2025-01-24 | GSE287602 | GEO

Transcriptome sequencing after MAGOHB knockdown in MAGOH-deleted or non-deleted cancer cells

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Pancreatic Cancer HPAF/CD18 cells: Ecd knockdown vs Scrambled

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Project description:MacroH2A2 knockdown vs control ATAC-seq

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RNA-seq CELSR2 knockdown vs NTC

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KPC vs Wild Type Pancreatic Fibroblasts

Project description:These experiments aim to determine global gene expression patterns in WT vs KPC isolated pancreatic fibroblasts

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MCT4 knockdown in pancreatic cancer cells

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