Project description:This SuperSeries is composed of the following subset Series: GSE23859: Regional Immune Response to Trichostrongylis colubriformis - Gene Expression during the Development of Immunity GSE23863: Regional Immune Response to Trichostrongylis colubriformis - Gene Expression during Challenge of Immunized Sheep Refer to individual Series
Project description:The bryophyte was collected and dried. Aqueous extract was prepared and different concentration of the extracts were treat against Ascardia galli, chicken nematode and Trichostrongylus, sheep nematode. Control was maintained for both. The control and treated samples were further processed to study the gene expression by isolating and sequencing mRNA using Illumina next-gen sequencing.
Project description:Immunity to intestinal helminth infections has been well studied, but the mechanism of helminth killing prior to expulsion remains unclear. Here we identify epithelial-cell-derived phospholipase A2 group 1B (PLA2g1B) as a host-derived endogenous anthelmintic. PLA2g1B is elevated in resistant mice and is responsible for killing tissue-embedded larvae. Despite comparable activities of other essential type-2-dependent immune mechanisms, Pla2g1b-/- mice failed to expel the intestinal helminths Heligmosomoides polygyrus or Nippostrongylus brasiliensis. Expression of Pla2g1b by epithelial cells was dependent upon intestinal microbiota, adaptive immunity, and common-gamma chain-dependent signaling. Notably, Pla2g1b was downregulated in susceptible mice and inhibited by IL-4R-signaling in vitro, uncoupling parasite killing from expulsion mechanisms. Resistance was restored in Pla2g1b-/- mice by treating infective H. polygyrus L3 larvae with PLA2g1B, which reduced larval phospholipid abundance. These findings uncover epithelial-cell-derived Pla2g1b as an essential mediator of helminth killing, highlighting a previously overlooked mechanism of anti-helminth immunity.
2018-08-13 | MTBLS523 | MetaboLights
Project description:Gene networks associated with host resistance to parasitic infections in sheep
