Project description:The goal of this study is to get the profile of the OVA-treated MutuDC transcriptome. Based on the dada of transcriptome, we processed our sgRNA sequencing data of genome-wide CRISPR/Cas9 screening. Briefly, after we have obtained the enriched gene list from our sgRNA sequencing, we excluded the genes undetected in OVA-treated MutuDC transcriptome to further refine our screening gene list.
Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goal of this study is to compare the transcriptome of OT-1 cells during priming (3 days after infection) and during effector phase ( 7 days after infection) in ODC-OVA mice after LCMV-OVA and Lm-OVA infection
Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goal of this study is to compare the transcriptome of CNS-infiltrating OT-1 WT and Tox-deficient cells during effector phase (7 days after infection with LCMV-OVA)
Project description:Activation of the Alpha kinase 1 (ALPK1) is a promising strategy to be developed as a next-generation cancer immunotherapy. We used single cell RNA sequencing (scRNA-seq) to analyze the ALPK1 activation-triggered antitumour immune responses within the tumour microenvironment of B16F10-OVA tumours treated with PBS or UDSP-Hep.
Project description:We treated the RKO cells with siSRSF1 or control siRNA for 48 hours. Then we extracted the RNAs and performed the next generation sequencing. By comparing sequcing data from siSRSF1 and contron siRNA treated samples, we profiled the gene expression regulated by siRNA.
Project description:We treated the RKO cells with siCHERP or control siRNA for 72 hours. Then we extracted the RNAs and performed the next generation sequencing. By comparing sequcing data from siCHERP and contron siRNA treated samples, we profiled the gene expression regulated by siRNA.
Project description:The purpose of this study is to determine the proportion of patients diagnosed with Lynch syndrome in colorectal cancer patients with the loss of staining by immunohistochemistry (IHC) of any of the mismatch repair (MMR) proteins. Besides, this study aims to test the specificity and the sensitivity of detecting microsatellite instability (MSI) by next-generation sequencing, and to find out the consistency between IHC and MSI in colorectal cancer patients in China. In addition, researchers want to analyze the clinical characteristics and germline mutation of Lynch syndrome in Chinese population.
Project description:We treated the RKO cells with siSRSF2 or control siRNA for 48 hours.Then, we extracted RNAs and performed next generation sequencing. By comparing sequencing data from control and siSRSF2 samples, we profiled the alternative splicing events and gene expression regulated by SRSF2 in CRC.