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Comparison of genetic differences between Streptomyces scabies and turgidiscabies strains

ABSTRACT: Comparison of genetic differences between Streptomyces scabies and turgidiscabies strains

PROVIDER: PRJNA121531 | ENA |

REPOSITORIES: ENA

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Comparison of genetic differences between Streptomyces scabies and turgidiscabies strains

Project description:Bacteria isolated from potato scab lesions in Finland or northern Sweden were analyzed using microarrays, PCR, and sequencing. Data indicate wide genetic variability in pathogenicity islands among S.turgidiscabies and S.scabies strains. Thirteen Streptomyces scabies and turgidiscabies strains from two different growings, Streptomyces reticulisabiei reference strain DSM41804 and Streptomyces scabies reference strain ATCC49173 were hybridized. Data were analyzed in single channel mode.

2010-06-18 | E-GEOD-18665 | biostudies-arrayexpress

Streptomyces turgidiscabies

Project description:Streptomyces turgidiscabies overview

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Comparison of genetic differences between Streptomyces scabies and turgidiscabies strains

2010-02-01 | GSE18665 | GEO

Gene expression of Streptomyces turgidiscabies pathogenicity island

Project description:Gene expression in Streptomyces turgidiscabies pathogenicity island was studied after transferring cells into thaxtomin A inducing medium OBB using Agilent 60mer oligonucleotide array with probes designed for S.scabies and S.turgidiscabies. Gene expression study was used to confirm results of gene prediction.

2010-02-01 | GSE18375 | GEO

Streptomyces turgidiscabies Car8

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Streptomyces turgidiscabies

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Gene expression of Streptomyces turgidiscabies pathogenicity island

2010-05-15 | E-GEOD-18375 | biostudies-arrayexpress

Microarray-based comparison of genetic differences between strains of Streptomyces turgidiscabies with focus on the pathogenicity island.

Project description:The areas of the pathogenicity island (PAI) designated as 'colonization region' (CR) and 'toxicogenic region' (TR) [Lerat et al. (2009) Mol. Plant Pathol. 10, 579-585] contain genes required for virulence and phytoxin production, respectively, in Streptomyces spp. causing common scab on potatoes. The PAI was tested for genetic variability by microarray analysis in strains of S. turgidiscabies isolated from potatoes in Finland. The data revealed four types of PAI based on divergent CR and TR which occurred in different combinations. Only one PAI type was highly similar to S. scabies (strains 87.22 and ATTC49173). Using probes designed for the predicted genes of S. scabies, two gene clusters in S. scabies appeared to be similar to most strains of S. turgidiscabies and contained PAI genes corresponding to CR and TR. They were located approximately 5 Mb apart in the S. scabies genome, as compared with only 0.3 Mb in S. turgidiscabies Car8. Data from comparative genomic hybridization with probes designed for S. scabies genes and for the PAI of S. turgidiscabies were compared by multilocus cluster analysis, which revealed two strains of S. turgidiscabies that were very closely related at the whole-genome level, but contained distinctly different PAIs. The type strain of S. reticuliscabiei (DSM41804; synonymous to S. turgidiscabies) was clustered with S. turgidiscabies. Taken together, the data indicate wide genetic variability of PAIs among strains of S. turgidiscabies, and demonstrate that PAI is made up of a mosaic of regions which may undergo independent evolution.

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Streptomyces turgidiscabies strain:W2I16

Project description:Streptomyces turgidiscabies W2I16 genome sequencing

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Microarray-based species identification of Streptomyces spp. isolated from potato scab lesions in Norway

Project description:28 Streptomyces strains isolated from common scab lesions of potato tubers from a wide geographic range in Norway, were selected for microarray analysis. The selected strains were subjected to species identification by microarray, 16S phylogenetic analysis and PCR; and microarray-based comparative genome analysis. To our knowledge, this is the first report of S. turgidiscabies and S. europaeiscabiei in Norway.

2010-05-01 | GSE20487 | GEO

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