Project description:affy_meja_arabidopsis - We want to determine if the presence of C2 modifies the cell transcriptome and its response to methyl jasmonate. Comparison of control lines and transgenic lines expressing C2 in basal conditions and after treatment with 50uM MeJA for 10 hours. C2 lines are expressing geminivirus C2 under a 35S promoter. PR1-LUC have a luciferase transgene under the control of the promoter of PR1; since the plants are not induced, these are the control plants (resistant to kanamycin, but not expressing anything else) Keywords: gene knock in (transgenic),treated vs untreated comparison 12 arrays - ATH1
Project description:This study evaluates the transcriptome of Arabidopsis thaliana seedlings (Col-0 ecotype) treated with methyl jasmonate (MeJA) or with the salicylic acid analog benzothiadiazole (BTH).
Project description:affy_meja_arabidopsis - We want to determine if the presence of C2 modifies the cell transcriptome and its response to methyl jasmonate. Comparison of control lines and transgenic lines expressing C2 in basal conditions and after treatment with 50uM MeJA for 10 hours. C2 lines are expressing geminivirus C2 under a 35S promoter. PR1-LUC have a luciferase transgene under the control of the promoter of PR1; since the plants are not induced, these are the control plants (resistant to kanamycin, but not expressing anything else) Keywords: gene knock in (transgenic),treated vs untreated comparison
Project description:This study evaluates the transcriptome of Arabidopsis thaliana seedlings chronically exposed to the hormone Methyl Jasmonate (MeJA) or to the bacterial elicitor flg22 (a 22-amino acid peptide from flagellin). Treatments were performed under high and low phosphate availability using wild-type Col-0 plants and the phr1 phl1 mutant.
Project description:affy_l2bctv_ath - affy_l2bctv_ath - Geminivirus diseases are among the most important economic impact on crops, including beans, cassava, cotton, cucurbits, corn, peppers and tomatoes. Geminiviruses have a genome composed of one or two molecules of single-stranded DNA, depending on mono or bipartite. The BCTV genome consists of a single strand of DNA that contains six reading frames for proteins CP, V2, Rep, C2, Ren, and C4. To determine the mechanism by which C2 of BCTV induce genes, we performed transgenic Arabidopsis expressing BCTV C2. We are interesting to study the difference between the wild type and two mutants. -10 days old plants were grown in MS at 24 degrees in long day. We used the different lines: wt, L2.4 mutants and L2.5 mutants. We performed three repetitions each. Keywords: gene knock in (transgenic)
