Project description:Nitrogen starvation is an efficient environmental pressure used to increase lipid accumulation and oil droplet formation in microalgal cells. Various studies focused on metabolic changes occurring in microalgae in nitrogen starvation conditions, but the mechanisms at the basis of these changes are not completely understood. Between microalgae, green algae, with more than 7000 species growing in a variety of habitats, have been frequently studied for energy purposes, but also as source of bioactive extracts/compounds. In this study, de novo transcriptome of the green algae Tetraselmis suecica has been performed in order to (1) deeply study its response to nitrogen starvation, (2) to look for enzymes with antioxidant capacity and for polyketide synthases (PKSs), (3) if present, to evaluate if nutrient starvation can influence their expression levels.

2018-12-01 | GSE109461 | GEO

Tateyamaria

Project description:Tateyamaria sp. overview

| PRJNA961874 | ENA

The biosynthesis of nitrous oxide in the green algae Chlamydomonas reinhardtii

Project description:This data was generated to identify the molecular pathways responsible for nitrous oxide synthesis by the green algae Chlamydomonas reinhardtii, when supplied with nitrite under aerobic conditions (oxia). RNA samples were collected at three time points, 15 min, 3 hours, and 24 hours after the start of the experiment. The control and treatment groups were grown under the same conditions, except treatment group was supplied with 10mM nitrite at time 0. Illumina TruSeq stranded RNA libraries were synthesised from the resulting RNA before sequencing on a HiSeq2500 (125bp). The resulting sequence run generated 241,151,809 paired-end 125bp reads, of which 200,946,839 remained following quality filtering. The short data was mapped to the published genome and read counts were generated with HT-Seq count with the default settings. The raw read count data was analysed by DESeq2 in order to identify genes differentially expressed during nitrous oxide production.

2017-07-01 | GSE90609 | GEO

Identification of Cypermethrin Induced Proteins Changes in Green Algae by iTRAQ Quantitative Proteomics

Project description:Cypermethrin (CYP) is one of the most widely used pesticides in large scale for agricultural and domestic purpose and the residue often seriously affects aquatic system. Environmental pollutants induced protein changes in organisms could be detected by proteomics, leading to discovery of potential biomarkers and understanding of mode of action. While proteomics investigations of CYP stress in some animal models have been well studied, few reports about the effects of exposure to CYP on algae proteome were published. To determine CYP effect in algae, the impact of various dosages (0.001 µg/L, 0.01 µg/L and 1 µg/L) of CYP on green algae Chlorella Vulgaris for 24h and 96h were investigated by using iTRAQ quantitative proteomics technique. A total of 162 and 198 proteins were significantly altered after CYP exposure for 24h and 96h, respectively. Overview of iTRAQ results indicated that the influence of CYP on algae protein might be dosage-dependent. Functional analysis of differentially expressed proteins showed that CYP could induce protein alterations related to photosynthesis, stress responses and carbohydrates metabolism. This study provides a comprehensive view of complex mode of action of algae under CYP stress and highlights several potential biomarkers for further investigation of pesticides exposed plant and algae.

2016-03-14 | PXD003521 | Pride

Phototropin connects blue light perception to starch metabolism in green algae

Project description:In photosynthetic organisms light acts as an environmental signal to control their development and physiology, and as energy source to drive the conversion of CO2 into carbohydrates used for growth or storage. The main storage carbohydrate in green algae is starch, which accumulates during the day and is broken down at night to meet cellular energy demands. The signalling role of light quality in the regulation of starch accumulation remains unexplored. Here, we report that in the model green alga Chlamydomonas reinhardtii blue light perceived by the photoreceptor PHOTOTROPIN suppresses starch accumulation. To gain more insight into the molecular mechanism underlying the PHOT-mediated light perception and starch accumulation, we applied we applied mass spectrometry (MS)-based quantitative proteomics to compare WT and phot cells grown in asynchronous photoautotrophic conditions under low light.

2025-01-29 | PXD046943 | Pride

Tateyamaria sp. syn59

Project description:Tateyamaria sp. syn59 Genome sequencing and assembly

| PRJNA542057 | ENA

Sarcocystis neurona strain:SN3

Project description:Sarcocystis neurona strain:SN3 Genome sequencing and assembly

| PRJNA227351 | ENA