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Schizosaccharomyces pombe

ABSTRACT: Argonaute Surveillance and Dicer-Independent priRNAs TriggerRNAi and Heterochromatin Formation

PROVIDER: PRJNA122193 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR035947.fastq.gz	Fastqsanger.gz
	SRR035948.fastq.gz	Fastqsanger.gz
	SRR035949.fastq.gz	Fastqsanger.gz
	SRR035950.fastq.gz	Fastqsanger.gz
	SRR035951.fastq.gz	Fastqsanger.gz

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RNAi mediates post-transcriptional repression of gene expression in fission yeast Schizosaccharomyces pombe [Affymetrix]

Project description:RNA interference (RNAi) is a gene silencing mechanism conserved from fungi to mammals. Small interfering RNAs are products and mediators of the RNAi pathway and act as specificity factors in recruiting effector complexes. The Schizosaccharomyces pombe genome encodes one of each of the core RNAi proteins, Dicer, Argonaute and RNA-dependent RNA polymerase (dcr1, ago1, rdp1). Even though the function of RNAi in heterochromatin assembly in S. pombe is established, its role in controlling gene expression is elusive. Here, we report the identification of small RNAs mapped anti-sense to protein coding genes in fission yeast. We demonstrate that these genes are up-regulated at the protein level in RNAi mutants, while their mRNA levels are not significantly changed. We show that the repression by RNAi is not a result of heterochromatin formation. Thus, we conclude that RNAi is involved in post-transcriptional gene silencing in S. pombe. Total RNA from wild type, dcr1M-NM-^T and rpd1M-NM-^T cells; 3 biological replicates for each strain.

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RNAi mediates post-transcriptional repression of gene expression in fission yeast Schizosaccharomyces pombe [Illumina Genome Analyzer]

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RNAi mediates post-transcriptional repression of gene expression in fission yeast Schizosaccharomyces pombe [Illumina Genome Analyzer]

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