Project description:3 samples of R1, R2 and R3 bone marrow monocytes were compared from 3 biological replicates in 3 separate experiments. R1, R2 and R3 were sorted from triplicate experiments from pools of mice
Project description:We used microarrays to analyze gene expression changes in liver after treatment of rats with two compounds from drug development (R1, R2) to identify potential effects related to hepatotoxicity. The development cpds have been described in: Pierson et al., J Med Chem 52: 3855-62. R1 corresponds to 36, R2 corresponds to 38
Project description:Chromatin immuno-precipitation using anti-Flag (Sigma) antibodies in a U2OS stable cell line. Paired-end R1 and R2 reads are provided, but the processed (mapped) reads are from a single-end (R1 read only) mapping.
Project description:Mononuclear phagocytes have important functions in regulating tissue injury and regeneration after AKI. We tested the hypothesis that kidney resident macrophages (R2) and infiltrative mononuclear phagocytes (R1) exist in distinct transcriptional subsets that are resolvable by single cell RNA sequencing. A further objective was to identify transcriptional signatures for those subsets. By cell sorting R2 and R1 cells from quiescent and injured mouse kidneys, we found remarkable heterogeneity among intrarenal innate immune cells. We define four subsets of dendritic cells and six subsets of R2 cells and specify differentially expressed genes that identify these cell types. Additionally, we observed subset-specific transcriptional responses to AKI. R2 cells, which express surface phenotype homogeneity, adopt distinct transcriptional programs in the steady state and after injury. Expression of MHC class II and invariant chain genes are decreased in a predominant subset of R2 cells after AKI. In contrast, other macrophage lineage-defining genes such as C1qa, b, and c, are expressed at similar levels across R2 cell subsets and their expression does not change in response to injury. This study has allowed us to identify R2 and R1 subsets that may prove to be therapeutic targets for inhibiting damaging inflammation and fibrosis or promoting tissue regeneration.
Project description:We used microarrays to analyze gene expression changes in liver after treatment of rats with two compounds from drug development (R1, R2) to identify potential effects related to hepatotoxicity. Experiment Overall Design: Compound R1 or R2, or vehicle control, was given for either 24h or 2 weeks (336h) at different doses.
Project description:The experiment aims to identify miRNAs that are regulated in response to TRAIL R1 si-RNA and TRAIL R2 si-RNA. The overall aim of the study was to identify mechanisms by which TRAIL death receptors may contribute to malignancy via inhibition of let-7 maturation.
Project description:The experiment aims to identify miRNAs that are regulated in response to TRAIL R1 si-RNA and TRAIL R2 si-RNA. The overall aim of the study was to identify mechanisms by which TRAIL death receptors may contribute to malignancy via inhibition of let-7 maturation. Comparison of TRAIL R1 si-RNA vs. control si-RNA and comparison of TRAIL R2 si-RNA vs. control
Project description:Interventions: Case series:Preoperative apatinib combined with S-1, for the maintenance of postoperative S-1
Primary outcome(s): Total resection rate (R0 / R1 / R2);Two-year disease-free survival rate;Safty;PFS;PFS
Study Design: Case series
