Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA122647 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The Human Genome Project promised to promote large-scale functional analyses necessary for the diagnosis and treatment of complex diseases. Here we demonstrate an integrated approach to the study of a transcriptional regulatory cascade involved in the progression of breast cancer and we identify a protein associated with disease progression. Using chromatin immunoprecipitation and genome tiling arrays, whole genome mapping of transcription factor binding sites was combined with gene expression profiling to identify genes involved in the proliferative response to estrogen (E2). Using RNA interference, selected ERa and c-Myc gene targets were knocked down to identify mediators of E2-stimulated cell proliferation. Tissue microarray screening revealed that high expression of an epigenetic factor, the estrogen-inducible histone variant H2A.Z, is significantly associated with lymph node metastasis and decreased breast cancer survival. Detection of H2A.Z levels independently increased the prognostic power of biomarkers currently in clinical use. This integrated approach has accelerated the identification of a molecule linked to breast cancer progression, has implications for diagnostic and therapeutic interventions, and can be applied to a wide range of cancers. Keywords: treated vs. untreated
2008-02-28 | GSE10618 | GEO
Project description:The Human Genome Project promised to promote large-scale functional analyses necessary for the diagnosis and treatment of complex diseases. Here we demonstrate an integrated approach to the study of a transcriptional regulatory cascade involved in the progression of breast cancer and we identify a protein associated with disease progression. Using chromatin immunoprecipitation and genome tiling arrays, whole genome mapping of transcription factor binding sites was combined with gene expression profiling to identify genes involved in the proliferative response to estrogen (E2). Using RNA interference, selected ERa and c-Myc gene targets were knocked down to identify mediators of E2-stimulated cell proliferation. Tissue microarray screening revealed that high expression of an epigenetic factor, the estrogen-inducible histone variant H2A.Z, is significantly associated with lymph node metastasis and decreased breast cancer survival. Detection of H2A.Z levels independently increased the prognostic power of biomarkers currently in clinical use. This integrated approach has accelerated the identification of a molecule linked to breast cancer progression, has implications for diagnostic and therapeutic interventions, and can be applied to a wide range of cancers. MCF7 cells treated with E2 (10nM) or Ctrl (vehicle) for 4, 12 or 24 hours. Each hormone treatment time was performed at least three times and hybridizations included dye swaps.
2010-06-25 | E-GEOD-10618 | biostudies-arrayexpress
Project description:Transcriptome of lsm8-1 mutant using whole genome tiling arrays
| PRJNA171255 | ENA
Project description:Mapping regulatory variation in sensory neurons using IPS lines from the HIPSCI project
2020-02-06 | E-ENAD-33 | ExpressionAtlas
Project description:Identification of chicken Interferon Stimulated Genes (ISGs) using Affymetrix GeneChip Chicken Genome Arrays
2015-07-14 | E-MTAB-3711 | ExpressionAtlas
Project description:Recent advances in multiple whole genome technologies provide unprecedented opportunities to profile epigenomic signatures in cancer cells. Previously we used a human gene promoter tiling microarray platform to identify genome-wide DNA methylation changes in a cell line model of breast cancer metastasis. Interestingly, the clustered nature of epigenetic targets that we identified, along with our concurrent karyotype analyses, have now led us to hypothesize that complex genomic alterations in cancer cells (deletions, translocations and ploidy) may be superimposed over promoter-specific methylation events that are responsible for gene-specific expression changes. This SuperSeries is composed of the SubSeries listed below.
2009-07-03 | GSE15619 | GEO
Project description:VEGF189 overexpression in breast cancer cells delays metastasis
2025-01-24 | E-GEOD-37911 | ExpressionAtlas
Project description:Gene expression profiling of Fowlpox knock-out mutant viruses using Affymetrix GeneChip Chicken Genome Arrays
2018-10-10 | E-MTAB-7276 | ExpressionAtlas
Project description:Gene expression profiling using exon arrays during interference with PPAR gamma signaling in thoracic aorta
2015-09-28 | E-GEOD-37195 | ExpressionAtlas
Project description:Transcript Mapping on Affymetrix ENCODE arrays for 3 different biological replicates of Placental Poly(A)+ RNA (each with either 2 or 3 technical replicates) Keywords = Transcript Mapping, Human, Affymetrix, Genome Tiling Arrays Keywords: other
2005-05-18 | GSE2671 | GEO