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Asparagine alleviates naphthalene-induced lens opacity in SRA cells

ABSTRACT: Asparagine alleviates naphthalene-induced lens opacity in SRA cells

PROVIDER: PRJNA1227507 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR32468638_1.fastq.gz	Fastqsanger.gz
	SRR32468638_2.fastq.gz	Fastqsanger.gz
	SRR32468639_1.fastq.gz	Fastqsanger.gz
	SRR32468639_2.fastq.gz	Fastqsanger.gz

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Asparagine alleviates naphthalene-induced lens opacity in SRA cells

Project description:Cataract is characterized by lens opacity, often leading to vision loss. The primary clinical treatment is lens replacement, which usually yields good results. Although cataract surgery is a common ophthalmic treatment, it still carries surgical risks, such as infection, bleeding, and dislocation of the intraocular lens. Therefore, researching drugs that can delay or treat cataract holds significant social and scientific importance. This study found that asparagine can improve lens opacification in rats exposed to naphthalene. Consequently, in vitro, SRA01/04 cells were treated with the naphthalene metabolite 1,2 - dihydroxynaphthalene (1,2 - DHN), and RNA - sequencing technology was employed to investigate the cellular mechanisms underlying the therapeutic effects of asparagine.Through systematic experiments and analyses, we have dissected the cellular changes following treatment with 1,2 - DHN, comprehensively presenting the molecular network affected thereby. Simultaneously, we have also paid particular attention to the signaling pathways activated by Asn during this process.

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Project description:Degradation of polycyclic aromatic hydrocarbons (PAHs) such as naphthalene by anaerobic microorganisms is poorly understood. Strain NaphS2, an anaerobic sulfate reducing marine delta-proteobacterium is capable of using naphthalene and the aromatic compound benzoate, as well as pyruvate, as an electron donors in the presence of sulfate. In order to identify genes involved in the naphthalene degradation pathway, we compared gene expression in NaphS2 during growth on benzoate vs. pyruvate, naphthalene vs. pyruvate, and naphthalene vs benzoate. For each experimental set, aRNA from NaphS2 was labelled Cy5 (experiment) or Cy3(control) with three biological replicates hybridized in duplicate. In addition, because of the size of the predicted genome of NaphS2, ORFs were divided into two separate array designs, designated set1 and set2, such that set1 and set2 represent two separate array designs (probe sets) to be treated separately in statistical analysis.

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