Project description:Gene methylation profiling of immortalized human mesenchymal stem cells comparing HPV E6/E7-transfected MSCs cells with human telomerase reverse transcriptase (hTERT)- and HPV E6/E7-transfected MSCs. hTERT may increase gene methylation in MSCs. Goal was to determine the effects of different transfected genes on global gene methylation in MSCs.

2011-08-25 | GSE23936 | GEO

Human iPS cells and fibroblasts

Project description:This SuperSeries is composed of the following subset Series: GSE18226: Expression data from human iPS cells and fibroblasts GSE18227: DNA methylation data from human iPS cells and fibroblasts Refer to individual Series

2009-10-31 | E-GEOD-18111 | biostudies-arrayexpress

Homo sapiens immortalized MSCs: KP vs. 3A6

2011-08-24 | E-GEOD-23936 | biostudies-arrayexpress

Homo sapiens

Project description:Expression data in human oral mucosa fibroblasts (hOFs), dermal fibroblasts, and hOFs-derived iPS cells

| PRJNA244636 | ENA

Methylation orofiling of human stem cell reprogramming by targeted bisulfite sequencing

Project description:We report a method for specific capture of an arbitrary subset of genomic targets for single molecule bisulfite sequencing, and for digital quantitation of DNA methylation at a single nucleotide resolution. We used targeted bisulfite sequencing to characterize the changes of DNA methylation during the de-differentiation of human fibroblasts into hybrid stem cells, and into induced pluripotent stem cells. We compared the methylation level of approximately 66,000 CpG sites within 2020 CpG islands on chromosome 12, chromosome 20, and 34 selected regions. A total of 288 differentially methylated regions were identified between fibroblasts and pluripotent cells. Methylation cluster analysis revealed distinct methylation patterns between fibroblasts and pluripotent cells. Furthermore iPS cells are globally more methylated than human embryonic stem cells, which could be due to the reprogramming process. This targeted bisulfite sequencing method is particularly useful for efficient and large-scale analysis of DNA methylation in organisms with large genomes. Experiment Overall Design: Comparison of DNA methylation on 2020 CpG islands and 34 other selected regions among eleven human ES, iPS and fibroblast lines.

2009-08-28 | E-GEOD-15007 | biostudies-arrayexpress

Homo sapiens

Project description:Incomplete DNA methylation underlies a transcriptional memory of somatic cells in human iPS cells

| PRJNA131167 | ENA