Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens

ABSTRACT: Human variation in PolII and NF-KappaB binding (RNA-seq study uninduced by TNF-alpha)

OTHER RELATED OMICS DATASETS IN: GSE19486

PROVIDER: PRJNA123911 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR038446.fastq.gz	Fastqsanger.gz
	SRR038447.fastq.gz	Fastqsanger.gz
	SRR038448.fastq.gz	Fastqsanger.gz
	SRR038449.fastq.gz	Fastqsanger.gz
	SRR038450.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 14

Similar Datasets

Homo sapiens

Project description:Human variation in PolII and NF-KappaB binding (RNA-seq study with TNF-alpha induced)

| PRJNA123947 | ENA

Homo sapiens

Project description:Human variation in PolII and NF-KappaB binding (ChIP-seq study with NF-KappaB)

| PRJNA123953 | ENA

Project description:Human variation in PolII and NF-KappaB binding

| PRJNA122275 | ENA

Human variation in PolII and NF-KappaB binding (RNA-seq study with TNF-alpha induced)

Project description:We examined genome-wide variation in transcription factor binding in different individuals and a chimpanzee using chromatin immunoprecipitation followed by massively-parallel sequencing (ChIP-Seq). The binding sites of RNA Polymerase II (Pol II) as well as a key regulator of immune responses, NFkB, were mapped in ten HapMap lymphoblastoid cell lines derived from individuals of African, European, and Asian ancestry, including a parent-offspring trio. We also mapped gene expression in all ten human cell lines for two treatment conditions: a) no treatment and b) following induction by TNF-alpha. Genome-wide comparison of Pol II and NF-KappaB binding in ten individuals. RNA-seq study with TNF-alpha treatment.

2010-03-22 | E-GEOD-19481 | biostudies-arrayexpress

Human variation in PolII and NF-KappaB binding (ChIP-seq study with Pol II)

Project description:Human variation in PolII and NF-KappaB binding (ChIP-seq study with Pol II)

| PRJNA123951 | ENA

Human variation in PolII and NF-KappaB binding (ChIP-seq study with NF-KappaB)

2010-03-22 | E-GEOD-19485 | biostudies-arrayexpress

PU.1 and C/EBPalpha synergistically program distinct response to NF-kappaB activation through establishing monocyte specific enhancers (expression data)

Project description:Unraveling the complexity of transcriptional programs coded by different cell types has been one of the central goals of cell biology. Using genome-wide location analysis, we examined how two different cell types generate different responses to the NF-kappaB signaling pathway. We showed that, after tumor necrosis factor-alpha (TNF-alpha) treatment, NF-kappaB p65 subunit binds to distinct genome locations and subsequently induces different subsets of genes in human monocytic THP-1 cells versus HeLa cells . Interestingly, the differential p65 binding in two cell types correlates with pre-existing cell-type specific enhancers prior to TNF-alpha stimulation, marked by histone modifications. We also found that two transcription factors, PU.1 and C/EBPalpha, appear to synergistically mediate enhancer creation and affect NF-kappaB target selection in THP-1 cells. In HeLa cells, co-expression of PU.1 and C/EBPalpha conferred TNF-alpha responsiveness to a subset of THP-1 specific NF-kappaB target genes. These results suggest that the diversity of transcriptional programs in mammalian cells arises, at least in part, from pre-existing enhancers that are established by cell specific transcription factors. We used Affymetrix microarray (GPL570) to obtain gene expression data for THP1 and HeLa cells before and after TNF-alpha treatment.

2011-03-15 | E-GEOD-26868 | biostudies-arrayexpress

Human variation in PolII and NF-KappaB binding (RNA-seq study uninduced by TNF-alpha)

2010-03-22 | E-GEOD-19466 | biostudies-arrayexpress

Human variation in PolII and NF-KappaB binding (ChIP-seq study with Pol II)

2010-03-22 | E-GEOD-19484 | biostudies-arrayexpress

PU.1 and C/EBPalpha synergistically program distinct response to NF-kappaB activation through establishing monocyte-specific enhancers (ChIP-chip data)

2011-03-15 | GSE26988 | GEO