Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA124011 | ENA |
REPOSITORIES: ENA
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Project description:This SuperSeries is composed of the following subset Series: GSE19482: Transcriptional responses of human monocyte-derived macrophages (HMDM) to lipopolysaccharide (LPS) GSE19490: Transcriptional responses of mouse BMM and TEPM to lipopolysaccharide (LPS) GSE19765: Transcriptional responses of human monocyte-derived macrophages (HMDM) to lipopolysaccharide (LPS) - Illumina arrays GSE19766: Transcriptional responses of mouse bone marrow-derived macrophages (BMM) to lipopolysaccharide (LPS) - Illumina arrays Refer to individual Series
2012-03-28 | E-GEOD-19492 | biostudies-arrayexpress
Project description:These microarrays were performed for use in a genome-wide scan for LPS-regulated genes in mouse macrophages, in order to construct a list of LPS-regulated genes for detailed interrogation on custom microarrays (see GSE19490 for custom array analysis). Mouse macrophages (bone marrow-derived macrophages, BMM) were stimulated with the TLR4 agonist, lipopolysaccharide, over a time course (0, 0.5, 2, 6, 24h) and analysed in biological duplicate by commercial Illumina microarray.
2012-03-28 | E-GEOD-19766 | biostudies-arrayexpress
Project description:This SuperSeries is composed of the following subset Series: GSE16180: Bone-marrow derived macrophages response to the Whipple's disease agent, Tropheryma whipplei GSE20207: Wild-type bone-marrow derived macrophages response to Escherichia coli lipopolysaccharide (LPS) GSE20208: Interleukin-16-knock-out bone-marrow derived macrophages response to Escherichia coli lipopolysaccharide (LPS) GSE20209: Interleukin-16-knock-out bone-marrow derived macrophages response to the Whipple's disease agent, Tropheryma whipplei Refer to individual Series
2010-11-08 | E-GEOD-20210 | biostudies-arrayexpress
Project description:Temporal analysis of bone marrow derived macrophages after 10 ug/ml lipopolysaccharide stimulation. C57BL6, C3H/ARC, BalbC and C3H/HeJ mouse strains analyzed. Each time point stimulated with 10ug/ml LPS from Salmonella minnesota for the time indicated. The RNA from each BMM stimulated time point was labeled with Cy3 and compared with a common reference (total RNA from C57BL6/J 17.5 embryo) labeled with Cy5.
2010-07-01 | E-GEOD-3148 | biostudies-arrayexpress
Project description:mRNA microarray analysis of bone marrow derived macrophages treated under four conditions, including Naïve (N). Bone marrow derived macrophages (BMDM) were derived from the bone marrow of mice and cultured in the presence of PAO, IFN-gamma, or lipopolysaccharide (LPS). Profiled groups include Naive, LPS, IFN, PAO.
2013-08-08 | GSE48609 | GEO
Project description:Interferon (IFN)γ and interleukin (IL)-4 are central regulators of T helper 1 (Th1) and T helper 2 (Th2) immune responses, respectively. Both cytokines have a major impact on macrophage phenotypes: IFNγ–priming and subsequent TLR4 activation induces so called classically activated macrophages that are characterized by pronounced pro-inflammatory responses, whereas IL-4–treated macrophages, commonly called alternatively activated, are known to develop enhanced capacity for endocytosis, antigen presentation, and tissue repair and are generally considered anti-inflammatory. Considering IL-4 as priming rather than activating stimulus, we now compared the TLR4–dependent global gene activation program in IFNγ– versus IL-4–pretreated mouse macrophages, which has rarely been studied so far. Although both cytokines frequently induced opposing effects on gene transcription, the subsequent activation of bone marrow-derived macrophages by lipopolysaccharide (LPS) produced a strong, priming dependent pro-inflammatory response in both macrophage types. For example, the production of key pro-inflammatory cytokines IL-6 and IL-12 was significantly higher in IL-4– versus IFNγ–primed macrophages and several cytokine genes, including Il19, Ccl17, Ccl22, Ccl24 and Cxcl5, were preferentially induced in alternatively primed and LPS activated mouse macrophages. In a subset of genes, including IL12a, IFNγ priming was actually found to suppress LPS–induced gene expression in a Stat1–dependent manner. Our data suggest that IL-4–priming is not per se anti-inflammatory but generates a macrophage that is “tissue protective” but still capable of mounting a strong inflammatory response after TLR4–dependent activation. Keywords: Gene expression profiling Gene expression was investigated in mouse bone marrow-derived macrophages (BMM). On day 7, BMM were stimulated with either IL-4 or IFNγ overnight (18h in total). LPS treatment was performed in primed and unprimed macrophages 4 h prior to harvesting. At least three independent experiments were performed for each condition.
2010-10-01 | E-GEOD-21895 | biostudies-arrayexpress
Project description:mRNA microarray analysis of bone marrow derived macrophages treated under four conditions, including Naïve (N). Bone marrow derived macrophages (BMDM) were derived from the bone marrow of mice and cultured in the presence of PAO, IFN-gamma, or lipopolysaccharide (LPS). Profiled groups include Naive, LPS, IFN, PAO. Compared each of the groups (PAO, LPS, IFN) with Naïve group.
2013-08-08 | E-GEOD-48609 | biostudies-arrayexpress
Project description:Bone marrow-derived macrophages, Unstimulated DMSO Bone marrow-derived macrophages, Unstimulated + I-BET (GSK525762A) Bone marrow-derived macrophages, LPS 1h DMSO Bone marrow-derived macrophages, LPS 1h + I-BET (GSK525762A) Bone marrow-derived macrophages, LPS 2h DMSO Bone marrow-derived macrophages, LPS 2h + I-BET (GSK525762A) Bone marrow-derived macrophages, LPS 4h DMSO Bone marrow-derived macrophages, LPS 4h + I-BET (GSK525762A) LPS (100 ng/mL) was purchased from Sigma. Bone Marrow-derived macrophages (BMDMs) were differentiated from C57BL/6 bone marrow using 5 ng/mL each of recombinant M-CSF and IL-3 (Peprotech) for 7 days as described (Jeffrey et al, Nature Immunology, 2006). 2 x10^6 BMDMs were treated with DMSO or 1 μM of I-BET for 30 minutes before the addition of LPS (100 ng/mL) for 1, 2 or 4h. Unstimulated control samples were incubated with I-BET only for 1 hour. 500 ng of total RNA from 3 independent samples per group was used to prepare biotin-labeled RNA using Ambion Illumina TotalPrep RNA Amplification Kit (Applied Biosystems) and hybridized to Illumina MouseRef-8 v2.0 expression BeadChip kits. The chips were scanned using Illumina BeadArray Reader.
2010-11-10 | GSE21764 | GEO
Project description:Bone marrow derived macrophages (BMM) total RNA from C3H/ARC mice extracted after stimulation by LPS at different time points. Each time point stimulated with 10ug/ml LPS from Salmonella minnesota for the time indicated. The RNA from each BMM stimulated time point was labeled with Cy3 and compared with a common reference (total RNA from C57BL6/J 17.5 embryo) labeled with Cy5. Keywords = LPS, macrophages, innate immunity
2010-06-05 | E-GEOD-324 | biostudies-arrayexpress
Project description:Bone marrow derived macrophages (BMM) total RNA from C3H/ARC mice extracted after stimulation by LPS at different time points. Each time point stimulated with 10ug/ml LPS from Salmonella minnesota for the time indicated. The RNA from each BMM stimulated time point was labeled with Cy3 and compared with a common reference (total RNA from C57BL6/J 17.5 embryo) labeled with Cy5. Keywords = LPS, macrophages, innate immunity
2010-06-05 | E-GEOD-326 | biostudies-arrayexpress