Project description:De novo shoot organogenesis (DNSO) is a commonly used pathway for plant biotechnology, and is a hormonally regulated process, where auxin and cytokinin coordinates suites of genes encoding transcription factors, general transcription factors, and RNA metabolism machinery genes. Here we report that silencing Arabidopsis thaliana CTD phosphatase-like 4 (CPL4RNAi), which increases phosphorylation level of RNA polymerase II (pol II) CTD, altered lateral root development and DNSO efficiency of the host plants, suggesting an importance of precise control of pol II activities during DNSO. Under standard condition, roots of CPL4RNAi lines produced no or few lateral roots. When induced by high concentration of auxin, CPL4RNAi lines failed to produce focused auxin maxima at the meristem of lateral root primordia, and produced fasciated lateral roots. By contrast, root explants of CPL4RNAi lines were highly competent for DNSO. Efficient DNSO of CPL4RNAi lines were observed even under 10 times less cytokinin required for wild type explants. Transcriptome analysis showed CPL4RNAi but not wild type explants expressed high levels of shoot meristem related genes during priming by high auxin/cytokinin ratio, and subsequent shoot induction with cytokinin. These results indicate that CPL4 functions as a repressor of the early stage of DNSO, during acquisition of competency by high auxin/cytokinin ratio, perhaps via regulation of pol II activities.

2020-09-19 | GSE104083 | GEO

Root proteome of ASPR1- deficient Arabidopsis thaliana compared to wt

Project description:Seedlings lacking the atypical aspartic protease encoded by the gene At2g03200 exhibited shorter primary roots and a pronounced reduction in the number of lateral roots. The protease was therefore name ATYPICAL ASPARTIC PROTEASE IN ROOTS 1 (ASPR1). This project compared the root proteome of a T-DNA insertion line lacking ASPR1 with wild type seedlings.

2019-02-25 | PXD009580 | Pride

Arabidopsis thaliana response to cytokinin in roots and shoots

Project description:This study aims to identify genes which are differentially expressed in root and/or shoot material in response to exogenous cytokinin. Roots and shoots were collected separately.

2015-08-01 | E-MTAB-4298 | biostudies-arrayexpress

Expression data from Arabidopsis thaliana - Pseudomonas interaction

Project description:Some soil bacteria promote plant growth, including Pseudomonas species. With this approach we detected significant changes in Arabidopsis genes related to primary metabolism that were induced by the bacteria. Pseudomonas G62 was applied to roots of 18 day-old Arabidopsis seedlings and the transcriptional profile of whole seedlings after 6 hours of treatment was analyzed.

2010-10-07 | E-GEOD-24552 | biostudies-arrayexpress

Gene expression changes following cytokinin treatment in hy5 Arabidopsis thaliana seedlings

Project description:Gene expression changes following cytokinin treatment in hy5 Arabidopsis thaliana seedlings

| PRJNA629520 | ENA

Arabidopsis thaliana

Project description:Time course IAA treatment Arabidopsis seedlings

| PRJNA248783 | ENA

Effect of high temperature on Arabidopsis thaliana root tips

Project description:Plant roots located in the upper soil layers are prone to experience high temperatures. To gain insight into the effect of high temperature on root development and functioning, we exposed five-day-old Arabidopsis thaliana seedlings grown on agar plates to 30 °C for 48 hours, and compared the gene expression profile in the root tip with that from seedlings that remained at 22 °C.

2024-02-07 | GSE254973 | GEO

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