Project description:Lacticaseibacillus paracasei strain:isolate from Guizhou white sour soup. | isolate:isolate from Guizhou white sour soup. Genome sequencing
Project description:Aspergillus were believed the dominant fungi in Post-fermentation Pu-erh tea (Pu-erh Shucha, PFPT), a well-known traditional Chinese tea with multiple healthful benefits. However, the functions of Aspergillus in PFPT fermentation lack deeply investigated. In this work, A. niger, A. tamarii and A. fumigatus were fermented on sun-dried green tea leaves, and these samples (marked as Asn, Ast and Asf) were analyzed by integration of proteomics and metabolomics methods. We identified 2035, 2210 and 1095 Unique proteins in Asn, Asf and Ast, and assigned majority of identified proteins to cellular process and metabolic process in GeneOntology (GO) analysis. Majority KEGG pathways enriched significantly belonged to Metabolism. Through dbCAN annotation, 232, 205 and 226 proteins were annotated to 103, 86 and 97 families or subfamilies of Carbohydrate-active enzymes (CAZymes) in Asn, Asf and Ast, respectively. CAZymes involved in degradation of cellulose, starch, lignin, pectin, xylan and xyloglucan were identified, such as endoglucanases, glucosidase, cellulase. We further identified enzymes hypothesized to hydrolysis, oxidization, polymerization, conversion, biosynthesis and degradation of polyphenols or benzenoids. Together, we found that Aspergillus. spp produced enzymes in tea leaves, which involved in the degradation of plant polysaccharides and metabolism of tea polyphenols.