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Expression data from rosette leaves of Arabidopsis thaliana wild type and rmpA/rmpB double transgenic exposed to formaldehyde

ABSTRACT: Expression data from rosette leaves of Arabidopsis thaliana wild type and rmpA/rmpB double transgenic exposed to formaldehyde

PROVIDER: PRJNA124657 | ENA |

REPOSITORIES: ENA

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Expression data from rosette leaves of Arabidopsis thaliana which has formaldehyde tolerance (rmpA/rmpB double transgenic) upon formaldehyde exposure

Project description:Formaldehyde is a toxic volatile organic compound and its mechanism of toxicity to plant has not yet been revealed. This experiment was designed to identify formaldehyde-responsible genes under exposure to low or high concentration of airborne formaldehyde for a short period of time. 7 weeks old Arabidopsis thaliana transformant (ecotype: Columbia) plants were exposed to gaseous formaldehyde at 1-2 ppm (low), 14-16 ppm (high), or less than 0.04 ppm (air control) at 24oC under light-condition for 150 minutes inside a chamber for formaldehyde exposure. Total RNA was isolated from rosette leaves of exposed plants and was applied to microarray analysis. We investigated into formaldehyde dose response on gene expression of Arabidopsis and tried to understand the toxic mechanisms of formaldehyde using an Affymetrix Arabidopsis genome array ATH-1.

2021-03-31 | GSE20865 | GEO

Expression data from rosette leaves of Arabidopsis thaliana wild type upon formaldehyde exposure

Project description:Formaldehyde is a toxic volatile organic compound and its mechanism of toxicity to plant has not yet been revealed. This experiment was designed to identify formaldehyde-responsible genes under exposure to low or high concentration of airborne formaldehyde for a short period of time. 7-weeks old Arabidopsis thaliana wild type (ecotype: Columbia) plants were exposed to gaseous formaldehyde at 1-2 ppm (low), 14-16 ppm (high), or less than 0.04 ppm (air control) at 24oC under light-condition for 150 minutes inside a chamber for formaldehyde exposure. Total RNA was isolated from rosette leaves of exposed plants and was applied to microarray analysis. We investigated into formaldehyde dose response on gene expression of Arabidopsis and tried to understand the toxic mechanisms of formaldehyde using an Affymetrix Arabidopsis genome array ATH-1.

2021-03-31 | GSE20863 | GEO

Expression data from rosette leaves of Arabidopsis thaliana wild type and rmpA/rmpB double transgenic exposed to formaldehyde

Project description:This SuperSeries is composed of the SubSeries listed below.

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Expression data from Arabidopsis rosette leaves

2014-10-09 | GSE62180 | GEO

miRNA expression data from A549 exposed to formaldehyde

Project description:We are investigating the miRNA expression profiles of human lung cells to gaseous formaldehyde We used microarrays to detail the global programme of miRNA expression upon response to formaldehyde

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Arabidopsis thaliana

Project description:Expression data from rosette leaves of Arabidopsis thaliana which has formaldehyde tolerance (rmpA/rmpB double transgenic) upon formaldehyde exposure

| PRJNA129629 | ENA

Expression data from Arabidopsis rosette leaves

Project description:The focus of this study was to identify changes in host gene expression induced by the transcription-dependent function of the viral AC2 protein, and induced by the interaction of AC2/C2 with SnRK1.2 (AtAKIN11). We used microarrays to identify changes in host gene expression induced by the transcription-dependent function of the geminivirus-encoded AC2 protein, and induced by the interaction of AC2 with a host serine-threonine kinase, SnRK1. Distinct classes of genes were up- and down regulated during this process. For experiment 1, Arabidopsis rosette leaves were infused with Agrobacterium capable of expressing full-length Cabbage leaf curl virus (CaLCuV) AC2 protein, a truncated version of AC2 lacking the C-terminal 29 amino acids (AC2del), or an empty vector control (530). In experiment 2, Arabidopsis rosette leaves were infused with Agrobacterium capable of expressing full-length Spinach curly top virus C2 protein, a truncated version of C2 lacking the C-terminal 29 amino acids (C2del), antisense (as) SnRK1.2 (AsAKIN11), or an empty vector control (530). Infused Arabidopsis rosette leaves were selected at 1, 2 or 3 days post-infusion for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous populations of plants at each time point in order to increase the temporal resolution of expression profiles. To that end, we isolated total RNA from four individual plants, for three independent sets of plants infused with the different constructs. This resulted in three independent samples per treatment per time point.

2014-10-09 | E-GEOD-62180 | biostudies-arrayexpress

Root and rosette leaves transcriptome of Arabidopsis Col-0 and nrt1.5 mutant

Project description:Differentially regulated genes in rosette leaves and roots of hydroponically grown Arabidopsis thaliana Col-0 and nrt1.5-5 mutant plants were identified by microarray analyses.

2017-02-28 | GSE60975 | GEO

Expression data from male cynomolgus macaques exposed to 6 ppm formaldehyde

Project description:Exogenous formaldehyde disrupts genomic/epigenomic profiles in the rodent nose and white blood cells (WBCs) related to inflammation and immune signaling, although it does not reach the circulating blood. We aimed to compare and contrast alterations in genomic signaling in the nose and circulating blood of non-human primates exposed to formaldehyde. We used microarrays to identify transcripts that were diffentially expressed in response to formaldehyde inhalation exposure.

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