Project description:Coprinopsis cinerea exhibits synchronised meiosis in the gill tissue of the fungus, which is 75% meiotic. The mushroom develops from a dikaryon, which contains two separate nuclei. These nucleifuse in the basidia (karyogamy). After karyogamy, the nuclei enter an extended meiotic prophase, in which pahcytene occurs at 6 hours post karyogamy (K+6). The tetrads produced by the second meiotic division are present 12 hours after karyogamy (K+12). To examine a comprehensive timecourse of meiosis in this organism, we took samples over a 15-hour period, 3 hours apart: K-3, K, K+3, K+6, K+9, K+12. Keywords: time course
Project description:Coprinopsis cinerea exhibits synchronised meiosis in the gill tissue of the fungus, which is 75% meiotic. The mushroom develops from a dikaryon, which contains two separate nuclei. These nucleifuse in the basidia (karyogamy). After karyogamy, the nuclei enter an extended meiotic prophase, in which pahcytene occurs at 6 hours post karyogamy (K+6). The tetrads produced by the second meiotic division are present 12 hours after karyogamy (K+12). To examine a comprehensive timecourse of meiosis in this organism, we took samples over a 15-hour period, 3 hours apart: K-3, K, K+3, K+6, K+9, K+12. Keywords: time course Four biological replicate samples of each timepoint were taken. Labelled cDNA was hybridized to arrays in a 2 channel reaction. The reference sample was a mixture of timepoint samples.
Project description:We have recently shown that the coprophilous model mushroom Coprinopsis cinerea transcribes a broad array of genes encoding defense proteins in the vegetative mycelium and fruiting bodies that target bacterial competitors and animal predators challenging the respective tissues of this fungus. In addition, we have demonstrated in previous work that two nematotoxic defense proteins from Coprinopsis, CGL1 and CGL2, were induced in vegetative mycelium challenged with the predatory nematode Aphelenchus avenae; however, the specificity and broadness of this response remained unclear. In order to resolve these issues, we sequenced the poly(A)-positive transcriptome of vegetative mycelium of C. cinerea confronted with nematode predation, hyphal mechanical damage or bacterial co-culture.
Project description:Mating compatibility in C. cinerea is controlled by two loci, A and B. Following fusion of undifferentiated hyphal cells, a complex program is initiated which results in the maintenance of one nucleus from each parent in every cell (the dikaryon). We identified downstream targets of the A locus (A on), the B locus (B on), and both loci (Aon Bon) using strains in which the two pathways are activated separately, and a dikaryotic strain. keywords: Cell type comparison
Project description:Coprinopsis cinerea vegetative dikaryotic mycelia compared to vegetative monokaryotic mycelia Vegetative dikaryon cDNA was comparitively hybridized with monokaryotic vegetative cDNA. Labelled cDNA was hybridized to arrays in a 2 channel reaction.
Project description:This SuperSeries is composed of the following subset Series: GSE37942: Comparison of gene expression during meiosis between wild-type and rad50-deficient strains of Coprinopsis cinerea GSE37943: Comparison of gene expression during meiosis between wild-type and msh5-deficient strains of Coprinopsis cinerea Refer to individual Series
Project description:Mating compatibility in C. cinerea is controlled by two loci, A and B. Following fusion of undifferentiated hyphal cells, a complex program is initiated which results in the maintenance of one nucleus from each parent in every cell (the dikaryon). We identified downstream targets of the A locus (A on), the B locus (B on), and both loci (Aon Bon) using strains in which the two pathways are activated separately, and a dikaryotic strain. keywords: Cell type comparison Four biological replicate samples of each experimental strain were taken. Labelled cDNA was hybridized to arrays in a 2 channel reaction. The reference sample was an unmated monokaryotic strain.
