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Optimized low-volume Plasmodium blood sample processing protocols for untargeted transcriptomics

ABSTRACT: Optimized low-volume Plasmodium blood sample processing protocols for untargeted transcriptomics

PROVIDER: PRJNA1251070 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR33148407_1.fastq.gz	Fastqsanger.gz
	SRR33148407_2.fastq.gz	Fastqsanger.gz
	SRR33148408_1.fastq.gz	Fastqsanger.gz
	SRR33148408_2.fastq.gz	Fastqsanger.gz
	SRR33148409_1.fastq.gz	Fastqsanger.gz

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Optimized low-volume Plasmodium blood sample processing protocols for untargeted transcriptomics

Project description:As the importance of transcriptional variation and regulation for Plasmodium becomes more apparent, advances for non-falciparum species are hindered by our reliance on natural infections to study parasite biology. Untargeted transcriptomic research is also complicated by low parasite densities and high proportions of human genetic material, highlighting the need for optimized sample processing protocols. In this study, we used a P. knowlesi culture diluted in whole blood as a mock P. vivax natural infection to compare white blood cell, rRNA-, and globin depletion methods and RNA-seq library preparation kits to create an optimized protocol for low-volume sample processing.

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