Project description:Nucleolus-associated DNA was isolated from MEF cells before and after conditional knock-out of UBF and hybridized against genomic DNA in biological replicates. Two different types of immortalized MEF cells were used. MEFs were immortalized by genetic depletion of p53, iMEFs were immortalized by transfection of the SV40 Tt antigen.
Project description:WT TR MEFs and Keap1–/– TR MEFs were established according to the following method. Keap1+/– mice were mated, and WT and Keap1–/– embryos were collected at embryonic day 13.5. Primary MEFs were immortalized by expression of SV40 T antigen. The immortalized MEFs were transformed by expression of HRAS G12V to generate WT TR MEFs and Keap1–/– TR MEFs.
