Project description:Genes that showed altered expression between wild type A909 and the isogenic Stp1 mutant were identified by microarray analysis Deletion mutants were examined for changes in gene expression
Project description:The commensal bacterium Streptococcus agalactiae is responsible for various infections in a wide variety of hosts including humans. Its broad spectrum of hosts shows its ability to acquire nutrients in variable conditions. The carbon catabolite repression allows bacteria to prioritize the uptake and the catabolism of the environmental sugars. In Gram-positive bacteria, CcpA (catabolite control protein A), a pleiotropic transcriptional regulator, plays a key role in catabolite repression. Studies have shown the involvement of carbon catabolite repression in the adaptation and stress resistance of pathogenic bacteria. The goal of this study is to determine the regulon and the role(s) of CcpA in the physiology and adaptation of S. agalactiae. To this aim, Streptococcus agalactiae strain A909 WT and its isogenic mutant ∆ccpA, obtained by allelic exchange were grown in filter-sterilized chemically defined medium (CDM) supplemented with 0,25% or 1% (w/v) of glucose. Their transcriptomes were compared under these two conditions by using RNA-seq.
Project description:Total RNA was isolated from mid-log phase Streptococcus agalactiae cells deficient in CopY (∆copY strain GU2857), grown in Todd-Hewitt broth (THB) medium and sequenced using Illumina NextSeq500.
Project description:Total RNA was isolated from mid-log phase Streptococcus agalactiae cells deficient in SczA (∆sczA strain GU2791), grown in Todd-Hewitt broth (THB) medium and sequenced using Illumina NextSeq500.
Project description:Total RNA was isolated from mid-log phase Streptococcus agalactiae cells deficient in CovR (∆covR strain GU2400), grown in Todd-Hewitt broth (THB) medium and sequenced using Illumina NextSeq500
Project description:Transcriptome analysis of Streptococcus agalactiae (group B Streptococcus) grown under control conditions or coincubated with serine hydroxamate to induce the bacterial stringent response
Project description:Genes that showed altered expression between wild type A909 and isogenic rgfC/A mutant were identified. A strain with a deletion in RgfC/A (SAK_1917/SAK_1918) was derived and microarray analysis was performed to identify genes regulated by the RgfC/A two component system. strain comparison, global regulation, two component system
