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Gene expression profiling of the ethanol tolerant strain HG228 in comparison to its parental strain Escherichia coli MG1655

ABSTRACT: Gene expression profiling of the ethanol tolerant strain HG228 in comparison to its parental strain Escherichia coli MG1655

PROVIDER: PRJNA126319 | ENA |

REPOSITORIES: ENA

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Gene expression profiling of the ethanol tolerant strain HG228 in comparison to its parental strain Escherichia coli MG1655

Project description:The goal of this experiment is to identify the pathways that are deregulated as part of an adaptive response to high levels of ethanol in the media. Ethanol tolerant cells (HG228) grown in minimal media plus 1.5% (v/v) ethanol were hybridized against E. coli MG1655 grown under similar conditions. The experiment was done in duplicates using biological replicates.

2010-06-15 | E-GEOD-21194 | biostudies-arrayexpress

Gene expression profiling of the ethanol tolerant strain HG228 in comparison to its parental strain Escherichia coli MG1655

Project description:The goal of this experiment is to identify the pathways that are deregulated as part of an adaptive response to high levels of ethanol in the media.

2010-06-15 | GSE21194 | GEO

Transcriptomic analysis of two ethanol-tolerant Saccharomyces cerevisiae clones obtained by evolutionary engineering

2016-03-01 | GSE78759 | GEO

Comparison of the transcriptome of the ethanol adapted yeast and control at 10% ethanol

Project description:In this study, using DNA microarray analysis, we compared the comprehensive expression profiles of two yeast trains, i.e, the previously obtained ethanol-adapted yeast strain and the parental strain as control (FY834), under the ethanol stress condition (YPD medium contained 10% ethanol). As a result, we identified certain genes and functional categories of the genes that are possible involved in growth under the ethanol stress condition.

2008-11-29 | GSE13757 | GEO

Expression data from ethanol-tolerant E. coli strain and wild type under ethanol stress

Project description:Cellular tolerance toward ethanol is a complex phenotype involved many genes, and hard to be improved by manipulating individual genes. We previously established exogenous global regulator IrrE mutants that confer Escherichia coli with significantly enhanced tolerance to stresses, including ethanol. In order to elucidate the mechanism for enhancement of ethanol tolerance in the mutants and to identify new genes and pathways that can be possible targets for engineering of ethanol tolerance, we carried out comparative transcriptomic and proteomic analyses with the representative strains E1 and E0 (harboring the ethanol-tolerant mutant E1 of IrrE and the wild type IrrE, respectively). The data from transcriptome analyses were deposited here. Cells of ethanol-tolerant mutant E1 and wild-type strain E0 were grown in 1.5% ethanol, and the samples for both of these two strains were prepared in triplicate with biological replicates

2012-04-30 | E-GEOD-30441 | biostudies-arrayexpress

Transcriptomic analysis of two ethanol-tolerant Saccharomyces cerevisiae clones obtained by evolutionary engineering

Project description:Evolutionary engineering strategy was used for selection of ethanol-tolerant Saccharomyces cerevisiae clones under gradually increasing ethanol stress levels. Clones B2 and B8 were selected based on their higher ethanol-tolerance and higher ethanol production levels. Whole genome microarray analysis was used for identifying the gene expression levels of these two evolved clones compared to the reference strain. Two evolved ethanol-tolerant strains B2 and B8, which were selected by evolutionary engineering under gradually increasing ethanol stress, were used for whole genome transcriptomic analysis in comparison with the reference strain. Cells were grown in yeast minimal media until they reach a final OD600 of 1. Following total RNA isolation, gene expression levels were analyzed using One-color microarray-based gene expression analysis (Agilent Technologies). Experiments were done in triplicates.

2016-03-01 | E-GEOD-78759 | biostudies-arrayexpress

Expression data from ethanol-tolerant E. coli strain and wild type under ethanol stress

2012-05-01 | GSE30441 | GEO

Transcriptomic responses of Clostridium (Ruminiclostridium) thermocellum ATCC27405 and ethanol tolerant strains to ethanol stress

Project description:Transcriptome profiles for Clostridium thermocellum ATCC 27405 wild type strain and two ethanol-adapted strains, E50A and E50C were generated to gain insights into ethanol tolerance. Details of the strains have been described, Shao X., et al. Appl Microbiol Biotechnol (2011) 92:641–652.

2018-03-31 | GSE77089 | GEO

Global protein expression profiles of laboratory isolated mutants of E. coli K12, MG1655

Project description:Resistance to the antibiotic mecillinam in Escherichia coli can be conferred by mutations in over 100 genes. In this study we compared the global protein expression levels in a number of mecillinam resistant mutants originating from E. coli MG1655 to the levels in the parental strain.

2019-07-29 | PXD007647 | Pride

Ethanol cellular defense induce unfolded protein response in yeast

Project description:Ethanol is a valuable industrial product and a common metabolite used by many cell types. However, this molecule produces high levels of cytotoxicity affecting cellular performance at several levels. In the presence of ethanol, cells must adjust some of their components, such as the membrane lipids to maintain homeostasis. In the case of microorganism as Saccharomyces cerevisiae, ethanol is one of the principal products of their metabolism and is the main stress factor during fermentation. Although many efforts have been made, mechanisms of ethanol tolerance are not fully understood and very little evidence is available to date for specific signaling by ethanol in the cell. This work studied two Saccharomyces cerevisiae strains, CECT10094 and Temohaya-26, isolated from flor wine and traditional fermentations respectively, which differ in ethanol tolerance, in order to understand the molecular mechanisms underlying the ethanol stress response and the reasons for different ethanol tolerance. The transcriptome was analyzed after ethanol stress and, among others, an increased activation of genes related with the unfolded protein response (UPR) and its transcription factor, Hac1p, was observed in the tolerant strain CECT10094. We observed that this strain also resist more UPR agents than Temohaya-26 and the UPR-ethanol stress correlation was corroborated observing growth of 15 more strains and discarding UPR correlation with other stresses as thermal or oxidative stress. Furthermore, higher activation of UPR pathway in the tolerant strain CECT10094 was observed using a UPR mCherry reporter. Finally, we observed UPR activation in response to ethanol stress in other S. cerevisiae ethanol tolerant strains as the wine strains T73 and EC1118.

2016-02-03 | GSE44863 | GEO

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