Project description:Investigation of gene expression level changes in Salmonella typhimurium LT2 TA100 upon exposure to C60, compared to unexposed controls. The mutations engineered into this strain make it susceptile to mutagenic compounds. The Salmonella typhimurium TA100 strain used in this study is further described in Pedersen P, Thomsen E, Stern RM. 1983. Detection by Replica Plating of False Revertant Colonies Induced in the Salmonella Mammalian Microsome Assay by Hexavalent Chromium. Environmental health perspectives 51: 227-230.
Project description:Investigation of gene expression level changes in Salmonella typhimurium LT2 TA100 upon exposure to C60, compared to unexposed controls. The mutations engineered into this strain make it susceptile to mutagenic compounds. The Salmonella typhimurium TA100 strain used in this study is further described in Pedersen P, Thomsen E, Stern RM. 1983. Detection by Replica Plating of False Revertant Colonies Induced in the Salmonella Mammalian Microsome Assay by Hexavalent Chromium. Environmental health perspectives 51: 227-230. A 4 x 72K array study using total RNA recovered from triplicate cultures of Salmonella typhimurium LT2 TA100 exposed to C60 and triplicate cultures of controls that were not exposed to C60. Each 72K array measures the expression level of 4,504 genes from Salmonella typhimurium LT2 with seven 45 to 60-mer probe pairs per gene.
Project description:Salmonellosis is one of the leading health problems worldwide. With the rise of drug resistance strains it has become imperative to identify alternative strategies. Naringenin, a flavonone, is present predominantly in grapefruit. Previously we have demonstrated that naringenin is potent inhibitor of cell-cell signaling. In the present study, we provide evidence that naringenin specifically represses 24 genes in the Salmonella pathogenicity island 1, and down-regulates 17 genes involved in flagellar and motility; thereby, attenuating virulence and cell motility, respectively. This is the first molecular evidence to demonstrate effect of naringenin on bacterial virulence and cell motility. One condition experiment, naringenin treated versus DMSO treated. Biological replicates: 3 control, 3 treatment, hybridized in dye-swapped design
Project description:Salmonellosis is one of the leading health problems worldwide. With the rise of drug resistance strains it has become imperative to identify alternative strategies. Naringenin, a flavonone, is present predominantly in grapefruit. Previously we have demonstrated that naringenin is potent inhibitor of cell-cell signaling. In the present study, we provide evidence that naringenin specifically represses 24 genes in the Salmonella pathogenicity island 1, and down-regulates 17 genes involved in flagellar and motility; thereby, attenuating virulence and cell motility, respectively. This is the first molecular evidence to demonstrate effect of naringenin on bacterial virulence and cell motility.
Project description:Investigation of whole genome gene expression level changes in a Salmonella enterica serovar Typhimurium UK1 delta-iacP mutant, compared to the wild-type strain. IacP is resoponsible for the secretion of virulence effector proteins via the type III secretion system, thereby contributing the virulence of S. Typhimurium. The mutants analyzed in this study are further described in Kim et al. 2011. Role of Salmonella Pathogenicity Island 1 Protein IacP in Salmonella enterica Serovar Typhimurium Pathogenesis. Infection and Immunity 79(4):1440-1450 (PMID 21263021).
Project description:FabR ChIP-chip on Salmonella enterica subsp. enterica serovar Typhimurium SL1344 using anti-Myc antibody against strain with chromosomally 9Myc-tagged FabR (IP samples) and wildtype strain (mock IP samples)