Project description:The DNA fragment of PtCAF1, one of the core component of PRC2, is fused with FlagHA and transformed into the macronuclear of Paramecium tetraurelia. The interacted proteins of PtCaf1 in Empty vector (EV) control and PtGTSF1 silenced cells were immunoprecipitated with anti-HA antibody, followed with mass spectrometry.
Project description:Differential transcriptome of Paramecium tetraurelia strain 51 undergoing RNAi by feeding against ICL7a (as a control) and RDR3 for nine days.
Project description:To study the role of structural maintenance of chromosome (SMC)4-1 and SMC4-2 during programmed genome rearrangement in Paramecium tetraurelia. Paramecium SMC4-1 and SMC4-2 was tagged with 3 FLAG and HA at its C-terminal separately. The recombinant plasmid was microinjected into macronuclear and used for co-immunoprecipitation and Mass spectrometry studies to identify interacting proteins of SMC4-1 and SMC4-2 that indicates the different functions between Paramecium SMC4s.
