Project description:Total RNA versus genomic DNA hybridization on custom arrays designed for all Debaryomyces hansenii genes Total RNA was collected in mid-log phase from Debaryomyces hansenii cells grown in rich medium (abbreviated CM, in house recipe). RNA was then converted to cDNA, Cy3-labeled and hybridized competitively against Cy5 labeled genomic DNA from Debaryomyces hansenii.
Project description:A comparison between the coculture of Debaryomyces hansenii with Yarrowia lipolytica and the monoculture of Debaryomyces hansenii, was carried out at two hours of culture (12h and 27h), in order to understand the metabolic changes induced in D. hansenii when grown in coculture with Y. lipolytica . The transcriptomic study was carried out using Agilent 8X15k slides with a custom oligoset designed by V. Loux using the Debaryomyces hansenii ORFeome extracted from genolevures database
Project description:In the frame of a multispecies project, Debaryomyces hansenii cells were treated with 0.5 mM sodium selenite. Cells were collected 10, 20, 30, 40, 50, 60, 70 and 80 minutes after the treatment and their transcriptomes were compared to those of mock-treated cultures. Four independent biological replicates were performed.
Project description:The present work studies the effect of Debaryomyces hansenii yeast-based products on physiological responses in Atlantic salmon exposed to a short-term stress. For this, a control fishmeal-based diet (CD) and experimental diets containing 0.1% of Debaryomyces hansenii were fed to fish for 8 weeks in fresh water. At the end of the feeding experiment, fish were exposed to 1-minute hypoxia stress and samples were collected post-stress
Project description:We found that the fungus, Debaryomyces hansenii (D. hansenii), is enriched in inflamed intestinal tissue from patients of Crohn's disease and its administration to mice impairs colonic wound healing in multiple models of colonic injury and repair. To understand the mechanism, we isolated bone marrow derived macrophages from mice and stimulated them in vitro with a pure isolate of D. hansenii. Total RNA was isolated at multiple time points and assayed for transcriptomic analysis.
