Project description:This study aims to identify genes which help to understand similar underlying mechanism in the response to shade and wounding in Arabidopsis thaliana plants.
Project description:Despite the importance of Nitric oxide (NO) in both plant and animal development, the regulatory and mechanism of NO function remain elusive. Here, we show that NO promotes Arabidopsis shoot stem cell differentiation via the RNA directed DNA Methylation (RdDM) pathway. Knocking out of the components of the RdDM pathway causes meristematic defects, and at least in part could block the stem cell and niche-specific genes responding to the NO signaling. Moreover, the homeobox WUSCHEL protein, known to control stem cell pool, has been found to interact with the ARGONAUTE protein, thus mediating NO signaling in the shoot apical meristem (SAM) is crucial for the stem cell fate. Our results reveal an important mechanism that NO is key to stem cell maintenance and differentiation.
Project description:Belonging to the Carmovirus family, Turnip crinkle virus (TCV) is a positive-strand RNA virus that can infect Arabidopsis. Most Arabidopsis ecotypes are highly susceptible to TCV, except for the TCV resistant line Di-17 derived from ecotype Dijon. Previous studies showed that many of the stress related genes have changed significantly after TCV infection. Besides the virus-triggered genes, small RNAs also play critical roles in plant defense by triggering either transcriptional and/or post-transcriptional gene silencing. In this study, TCV-infected wildtype Arabidopsis thaliana and dcl1-9 mutant plants were subjected to transcriptome and small RNA analysis to investigate the role of DCL1 in virus defense network.
Project description:Despite the importance of Nitric oxide (NO) in both plant and animal development, the regulatory and mechanism of NO function remain elusive. Here, we show that NO promotes Arabidopsis shoot stem cell differentiation via the RNA directed DNA Methylation (RdDM) pathway. Knocking out of the components of the RdDM pathway causes meristematic defects, and at least in part could block the stem cell and niche-specific genes responding to the NO signaling. Moreover, the homeobox WUSCHEL protein, known to control stem cell pool, has been found to interact with the ARGONAUTE protein, thus mediating NO signaling in the shoot apical meristem (SAM) is crucial for the stem cell fate. Our results reveal an important mechanism that NO is key to stem cell maintenance and differentiation.
Project description:Despite the importance of Nitric oxide (NO) in both plant and animal development, the regulatory and mechanism of NO function remain elusive. Here, we show that NO promotes Arabidopsis shoot stem cell differentiation via the RNA directed DNA Methylation (RdDM) pathway. Knocking out of the components of the RdDM pathway causes meristematic defects, and at least in part could block the stem cell and niche-specific genes responding to the NO signaling. Moreover, the homeobox WUSCHEL protein, known to control stem cell pool, has been found to interact with the ARGONAUTE protein, thus mediating NO signaling in the shoot apical meristem (SAM) is crucial for the stem cell fate. Our results reveal an important mechanism that NO is key to stem cell maintenance and differentiation.
