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Coccidioides immitis

ABSTRACT: Pathogenic fungus that is morphologically indistinguishable from Coccidioides posadasii both cause the disease coccidioidomycosis

PROVIDER: PRJNA12821 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR1292220_1.fastq.gz	Fastqsanger.gz
	SRR1292220_2.fastq.gz	Fastqsanger.gz
	SRR1292221_1.fastq.gz	Fastqsanger.gz
	SRR1292221_2.fastq.gz	Fastqsanger.gz

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Similar Datasets

Proteogenomic Re-annotation of Coccidioides posadasii strain Silveira

Project description:The aims of this study were to present modifications to the annotations of the genome of C. posadasii, one of two closely related species of Coccidioides, a dimorphic fungal pathogen that causes coccidioidomycosis, also called Valley Fever. Proteins present in lysates and filtrates of in vitro grown mycelia and parasitic phase spherules from C. posadasii strain Silveira were analyzed using a GeLC-MS/MS method.

2017-11-08 | PXD006489 | Pride

Gene expression in human fungal pathogen Coccidioides immitis changes as mycelia differentiate into spherules and mature

2013-07-11 | GSE44225 | GEO

Coccidioides posadasii

Project description:A dimorphic ascomycete causing coccidioidomycosis and morphologically indistinguishable from Coccidioides immitis.

| PRJNA10735 | ENA

Transcriptional analysis of Coccidioides immitis mycelia and spherules by RNA sequencing

Project description:Coccidioides immitis and posadasii are dimorphic fungi that transform from mycelia with internal arthroconidia in the soil to a tissue form known as a spherule. Genes that were highly up-regulated in young spherules include a spherule surface protein and iron and copper membrane transporters. Genes that are unique to Coccidioides spp. are also over-represented in this group, suggesting that they may be important for spherule differentiation. Enriched GO terms in up-regulated genes in young spherules include oxidation/reduction, response to stress and membrane proteins. Down-regulated genes are enriched for transcription factors, especially helix-loop-helix and C2H2 type zinc finger domain-containing proteins which is consistent with the dramatic change in transcriptional profile. Almost all genes that are up-regulated in young spherules remain up-regulated in mature spherules, but a small number of genes are differentially expressed in those two stages of spherule development. Mature spherules express more Hsp31, and amylase than young spherules and less tyrosinase. Some expression of transposons was detected and most of the differentially expressed transposons were up-regulated in spherules.

2021-04-01 | GSE171286 | GEO

The WOPR family protein Ryp1 is a key regulator of gene expression, development, and virulence in the thermally dimorphic fungal pathogen Coccidioides posadasii

Project description:Coccidioides spp. are fungal pathogens endemic to Southern California, Arizona, and other desert regions where they cause a spectrum of symptoms known as Valley Fever in otherwise healthy individuals. In the soil, Coccidioides grows in a hyphal form that produces vegetative spores (arthroconidia). When arthroconidia are inhaled by mammals they undergo an elaborate developmental transition consisting of germination, multiple rounds of nuclear division, and segmentation to form large spherules filled with vegetative endospores. Very little is understood about the molecular basis of spherule formation. Here we characterize the role of the conserved transcription factor Ryp1 in Coccidioides spherule formation. We show that Coccidioides ryp1 mutants are unable to form mature spherules, instead arresting at an early stage of spherulation. We analyze the transcriptional profile of wild-type and Δryp1 mutant cells under hyphal and spherule-promoting conditions, thereby defining a set of hyphal- or spherule-enriched transcripts that are dependent on Ryp1 for their expression. Ryp1-dependent transcripts include key virulence factors such as SOWgp, which encodes the spherule outer wall glycoprotein. In the mouse model of coccidioidomycosis, we find that the Δryp1 mutant is completely avirulent, indicating that Ryp1-dependent pathways are essential for the ability of Coccidioides to cause disease. Vaccination of C57BL/6 mice with live Δryp1 spores does not provide any protection from lethal C. posadasii intranasal infection, consistent with our findings that the Δryp1 mutant fails to make mature spherules. Taken together, this work identifies the first transcriptional regulator that drives mature spherulation and virulence in Coccidioides.

2022-03-03 | GSE178277 | GEO

Factors regulated by interferon gamma and hypoxia-inducible factor 1A contribute to responses that protect mice from Coccidioides immitis infection.

Project description:Mouse strains have been identified that are resistant (i.e. DBA/2) or susceptible (i.e. C57BL/6) to infection from pathogenic fungus Coccidioides immitis. However, the genetic and immunological basis for this difference has not been fully characterized. Microarray technology was used to identify genes that were differentially expressed in lung tissue between resistant DBA/2 and sensitive C57BL/6 mice after infection with C. immitis.

2012-09-10 | GSE40379 | GEO

Gene expression in human fungal pathogen Coccidioides immitis changes as mycelia differentiate into spherules and mature

Project description:Coccidioides immitis (C. immitis) is a dimorphic fungus that causes disease in mammals including human beings. It grows as a mycelium in the soil but differentiates into a pathogenic structure called a spherule in the host. We compared the transcriptome of C. immitis mycelia and day 2 and day 8 spherules grown in vitro using a custom custom oligonucleotide microarray from Nimblegen. C. immitis RS strain isolated from infected mice was grown on agar. Arthroconidia was harvested and inoculated into mycelial spores. RNA was extracted from spores at different days post-inoculation and hybridized to a custom Nimblegen array, in order to identify differentially expressed genes between mycelia and spherules.

2013-07-11 | E-GEOD-44225 | biostudies-arrayexpress

Coccidioides posadasii

Project description:Coccidioides posadasii in Caribe

| PRJNA438145 | ENA

Coccidioides immitis

Project description:Coccidioidomycosis increases its area of geographic risk: Molecular detection and isolation of Coccidioides immitis in Washington State from soil associated with recent human infection

| PRJNA245906 | ENA

AÂ Coccidioides posadasii CPS1Â deletion mutant is avirulent and protects mice from lethal infection

Project description:TheÂ CPS1Â gene was identified as a virulence factor in the maize pathogen, Cochliobolus heterostrophus. Hypothesizing that the homologous gene in Coccidioides posadasiiÂ (Cp) could be important for virulence, we created a deletion mutant,Â Îcps1, which was unable to cause disease in three strains of mice (C57BL/6, BALB/c, or the severely immunodeficient NOD-scid,Î³cnullÂ [NSG]). Only a single colony was recovered from one of 60 C57BL/6 mice following intranasal infections of up to 4400 spores. Following administration of very high doses (10,000 to 2.5 x 10^7Â spores) to NSG and BALB/c mice, spherules were observed in lung sections at time points from day 3 to day 10 post-infection, but nearly all appeared degraded with infrequent endosporulation. Although the role ofÂ CPS1Â in virulence is not understood, phenotypic alterations and transcription differences of at least 33 genes inÂ Îcps1Â vs. Cp is consistent with both metabolic and regulatory functions for the gene. The inÂ vitroÂ phenotype ofÂ Îcps1Â showed slower growth of mycelia with delayed and lower spore production compared to Cp, andÂ in vitro spherules were smaller. Vaccination of C57BL/6 or BALB/c mice with liveÂ Îcps1 spores either intranasally, intraperitoneally or subcutaneously resulted in over 95% survival with mean residual lung fungal burdens <1000 colony-forming units from an otherwise lethal Cp intranasal infection. Considering its apparently complete attenuation of virulence and the high degree of resistance to Cp infection when used as a vaccine,Â Îcps1Â is a promising vaccine candidate for preventing coccidioidomycosis in humans or other animals. Wild type and CPS1 deletion mutant strains of Coccidioides posadasii strain Silveira spherules grown for 48 hours in Converse medium at 38 degrees celsius in duplicate. RNAseq was performed on an Illumina HiSeq2000 (2x100 paired end).

2016-08-15 | E-GEOD-85364 | biostudies-arrayexpress

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