Project description:au14-03_bigun - bigun transcriptomics - What are the gene networks associated with BIGUN function during Hyaloperonospora arabidopsidis infection? - 5 weeks after germination of 12 seedlings of Arabidopsis thaliana Col-0,bigun 1 mutant and bigun 2 mutant genotypes(growth condition n°1),6 plants are infected by spraying a solution of Hyaloperonospora arabidopsidis spores,and 6 plants are left untreated,and all plants were cultivated under growth condition n°2.For each condition,leaves were sampled and pooled one week after infection.The experiment was repeated three times.
Project description:We used Arabidopsis full-genome microarrays to characterize plant transcript accumulations at different stages of infection with the biotrophic oomycete downy mildew pathogen, Hyaloperonospora arabidopsidis : initiation (< 1 dpi) and maintenance of infection (> 4 dpi).
Project description:We used Arabidopsis full-genome microarrays to characterize plant transcript accumulations at different stages of infection with the biotrophic oomycete downy mildew pathogen, Hyaloperonospora arabidopsidis : initiation (< 1 dpi) and maintenance of infection (> 4 dpi). In two independent experiments, cotyledons from the ecotype Wassilewskija (WS) were inoculated with water, or with Hyaloperonospora arabidopsidis to establish a compatible interaction. Affymetrix ATH1 microarrays were used to profile Arabidopsis transcript accumulations at the initiation (mixed samples at 8 and 24 hours post inoculation, hpi; early stage) and maintenance (mixed samples at 4 and 6 days post inoculation; late stage) of the compatible interaction.
