Project description:Brain metastasis (BM) can affect up to 25% of non-small cell lung cancer (NSCLC) patients during their lifetime. Efforts to characterize patients that will develop BM have been fairly disappointing. Small non-coding microRNAs (miRNAs) regulate the expression of target mRNAs by repressing their translation or regulating their sequence-specific degradation. miRNAs play a role in regulating a variety of targets and, consequently, multiple pathways, which makes them a powerful tool to be exploited for early detection of disease, risk assessment, and prognosis. In this study, we investigated miRNAs that may serve as biomarkers to differentiate between NSCLC patients with and without BM. miRNA microarray profiling was performed on samples from clinically matched NSCLC from patients with BM (BM+) and without BM (BM-). miR-328 and miR-330-3p were able to correctly classify BM+ vs. BM- patients. Gene expression analysis comparing NSCLC parental and stably transfected miR-328 cells identified several significantly differentially-expressed genes, whose expression may be directly or indirectly regulated by miR-328.
2011-02-16 | GSE23019 | GEO
Project description:Differentially expressed miRNAs in lung spheroid-derived cancer stem cells
Project description:The nCounter technology (Nanostring Technology) was used for the characterization of the repertoire of miRNAs expressed in CICs (initiating cells) and FBS (differentiated tumor cell) colorectal cancer cell lines. The Nanostring miRNA panel V3 (including ~800 targets) was run on all samples. The class comparison of the profile of miRNAs in CRC-CIC vs. FBS tumor cells led to the identification of N=57 differentially expressed miRNAs (p≤0.05). Most of the miRNAs were found to be upregulated in CICs vs. FBS cell lines, including the N=11 top scored differentially expressed (p<0.02, miRNAs -299, -15a, -210, -196a, -362, -378d, -3144, -4461, -4488 and let-7d). Pathway Analysis performed on target genes for the miRNAs, showing their involvement in tumorigenic functions, such as cell cycle, migration, development and proliferation and the regulation of EMT.
