ABSTRACT: mRNA microarray analysis on young adult Caenorhabditis elegans, Drosophila melanogaster, and Danio rerio exposed to benzene, toluene, and formaldehyde
Project description:This SuperSeries is composed of the following subset Series: GSE23013: mRNA microarray analysis on young adult Caenorhabditis elegans exposed to benzene, toluene, formaldehyde and a BTF mix GSE24845: mRNA microarray analysis on young adult Drosophila exposed to benzene, toluene, and formaldehyde GSE24846: mRNA microarray analysis on young adult zebrafish exposed to benzene, toluene, formaldehyde and a BTF mix Refer to individual Series
Project description:In order to evaluate the identification of genes and pathways, the global gene expression profiles were assessed in response to three chemicals (benzene (B), toluene (T), formaldehyde (F)) individually and as a BTF mix on the soil nematode, Caenorhabditis elegans. We performed whole genome DNA microarray experiments with subsequent quantitative analysis conducted on selected genes. We used synchronized C. elegans populations exposed to B, T, F or a BTF mix, and whole genome microarrays to screen for global changes in C. elegans transcription profiles. Young adults of C. elegans were selected for RNA extraction and hybridization on Affymetrix microarrays.
Project description:In order to evaluate the identification of genes and pathways, the global gene expression profiles were assessed in response to three chemicals (benzene (B), toluene (T), formaldehyde (F)) individually and as a BTF mix on the soil nematode, Caenorhabditis elegans. We performed whole genome DNA microarray experiments with subsequent quantitative analysis conducted on selected genes.
Project description:In order to evaluate the identification of genes and pathways, the global gene expression profiles were assessed in response to three chemicals (benzene (B), toluene (T), formaldehyde (F)) on Drosophila melanogaster. We performed whole genome DNA microarray experiments with subsequent quantitative analysis conducted on selected genes. We used young adults of D. melanogaster exposed to B, T, or F, and whole genome microarrays to screen for global changes in D. melanogaster transcription profiles. Young adult flies were selected for RNA extraction and hybridization on Affymetrix microarrays.
Project description:In order to evaluate the identification of genes and pathways, the global gene expression profiles were assessed in response to three chemicals (benzene (B), toluene (T), formaldehyde (F)) on Drosophila melanogaster. We performed whole genome DNA microarray experiments with subsequent quantitative analysis conducted on selected genes.
Project description:The effects of the aromatic hydrocarbons benzene and toluene on Nitrosomonas europaea, a nitrifying bacterium that plays an important role in the removal of nitrogen from wastewater treatment plants, were studied in batch reactors. Exposure to 20 M toluene and 40 M benzene resulted in a 50% reduction in nitrifying activity after 1 h. However, Affymetrix microarray experiments detected no significant changes in gene expression in toluene exposed cells. Cells exposed to benzene were found to up-regulate a gene cluster (NE 1545 - NE 1551). This gene cluster appears to be involved with fatty-acid metabolism, lipid and membrane protein biosynthesis. TEM experiments reveal that cells exposed to benzene decrease the thickness of their membrane and the membrane becomes more structured. Keywords: stress response, benzene, toluene
Project description:In order to evaluate the identification of genes and pathways, the global gene expression profiles were assessed in response to three chemicals (benzene (B), toluene (T), formaldehyde (F)) individually and as a BTF mix on zebrafish. We performed whole genome DNA microarray experiments with subsequent quantitative analysis conducted on selected genes. We used synchronized zebrafish populations exposed to B, T, F or a BTF mix, and whole genome microarrays to screen for global changes in zebrafish transcription profiles. Young adults of zebrafish were selected for RNA extraction and hybridization on Affymetrix microarrays.
